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Anti-ARRB1 Antibody (3Y284) 是一种 Rabbit 抗体,靶向 ARRB1。Anti-ARRB1 Antibody (3Y284) 可用于 WB,ICC/IF,IHC,IP,FCM。
Anti-ARRB1 Antibody (3Y284) 是一种 Rabbit 抗体,靶向 ARRB1。Anti-ARRB1 Antibody (3Y284) 可用于 WB,ICC/IF,IHC,IP,FCM。
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
50 μL | ¥ 1,495 | 5日内发货 | |
100 μL | ¥ 2,495 | 5日内发货 |
产品描述 | Anti-ARRB1 Antibody (3Y284) is a Rabbit antibody targeting ARRB1. Anti-ARRB1 Antibody (3Y284) can be used in WB,ICC/IF,IHC,IP,FCM. |
别名 | β-Arrestin-1, Beta-Arrestin-1, Arrestin β-1, Arrestin Beta-1, ARRB1, ARR1 |
Ig Type | IgG |
交叉反应 | Human,Mouse,Rat |
验证活性 | 1. Western blot analysis of beta Arrestin 1 on different lysates using anti-beta Arrestin 1 antibody at 1/1,000 dilution. Positive control: Lane 1: PC12, Lane 2: Jurkat. 2. Immunohistochemical analysis of paraffin-embedded human lung tissue using anti-beta Arrestin 1 antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-beta Arrestin 1 antibody. Counter stained with hematoxylin. 4. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-beta Arrestin 1 antibody. Counter stained with hematoxylin. 5. Immunohistochemical analysis of paraffin-embedded mouse lung tissue using anti-beta Arrestin 1 antibody. Counter stained with hematoxylin. 6. Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-beta Arrestin 1 antibody. Counter stained with hematoxylin. 7. ICC staining beta Arrestin 1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 8. ICC staining beta Arrestin 1 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 9. ICC staining beta Arrestin 1 in PC12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 10. Flow cytometric analysis of Hela cells with beta Arrestin 1 antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody. |
应用 | |
推荐剂量 | WB: 1:1000-2000; IHC: 1:50-100; ICC/IF: 1:50-200; FCM: 1:50-100 |
抗体种类 | Monoclonal |
宿主来源 | Rabbit |
构建方式 | Recombinant Antibody |
纯化方式 | ProA affinity purified |
性状 | Liquid |
缓冲液 | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
研究背景 | The members of the G protein coupled receptor family are distinguished by their slow transmitting response to ligand binding. These seven transmembrane proteins include the adrenergic, serotonin and dopamine receptors. The effect of the signaling molecule can be excitatory or inhibitory depending on the type of receptor to which it binds. Members of theβ-Arrestin family regulate receptor binding to G proteins. β-Arrestins have been found to be located at postsynaptic sites, where they are thought to act in concert withβARK (βARK1, also designated GRK 2, orβARK2, also designated GRK 3 to regulate G protein-coupled neurotransmitter receptors. Expression ofβ-Arrestin-1 and b-Arrestin-2 is seen predominantly in spleen and neuronal tissues. It has been shown thatβ-Arrestin-1 expression is modulated by intracellular cAMP, which may be a novel mechanism for the regulation of receptor-mediated responses. |
偶联 | Unconjugated |
免疫原 | Recombinant Protein |
Uniprot ID |
分子量 | Theoretical: 50 kDa. |
储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
运输方式 | Shipping with blue ice. |
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