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Anti-Arp3/ACTR3 Antibody (1Q436)
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产品编号 TMAC-00273
别名 ARP3 actin-related protein 3 homolog (yeast), ARP3
Anti-Arp3/ACTR3 Antibody (1Q436) 是一种 Rabbit 抗体，靶向 Arp3/ACTR3。Anti-Arp3/ACTR3 Antibody (1Q436) 可用于 WB,ICC,IF,IHC,FCM。
Anti-Arp3/ACTR3 Antibody (1Q436)
Anti-Arp3/ACTR3 Antibody (1Q436)
还可以产品编号 TMAC-00273 别名 ARP3 actin-related protein 3 homolog (yeast), ARP3
Anti-Arp3/ACTR3 Antibody (1Q436) 是一种 Rabbit 抗体，靶向 Arp3/ACTR3。Anti-Arp3/ACTR3 Antibody (1Q436) 可用于 WB,ICC,IF,IHC,FCM。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,490 | 5日内发货 | |
| 100 μL | ¥ 2,485 | 5日内发货 |
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抗原信息
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| 产品描述 | Anti-Arp3/ACTR3 Antibody (1Q436) is a Rabbit antibody targeting Arp3/ACTR3. Anti-Arp3/ACTR3 Antibody (1Q436) can be used in WB,ICC,IF,IHC,FCM. |
| 别名 | ARP3 actin-related protein 3 homolog (yeast), ARP3 |
| Ig Type | IgG |
| 克隆号 | 1Q436 |
| 交叉反应 | Human,Mouse,Rat |
| 验证活性 | 1. Western blot analysis of Arp3 on different lysates using anti-Arp3 antibody at 1/500 dilution.Positive control:Lane 1: A431, Lane 2: Mouse placenta, Lane 3: Mouse thymus. 2. Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-Arp3 antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-Arp3 antibody. Counter stained with hematoxylin. 4. Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue using anti-Arp3 antibody. Counter stained with hematoxylin. 5. Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-Arp3 antibody. Counter stained with hematoxylin. 6. ICC staining Arp3 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 7. ICC staining Arp3 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 8. ICC staining Arp3 in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 9. Flow cytometric analysis of HL-60 cells with Arp3 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow).Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody. |
| 应用 | |
| 推荐剂量 | WB: 1:500-1000; IHC: 1:50-200; ICC: 1:50-200; FCM: 1:50-100 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Rabbit |
| 构建方式 | Recombinant Antibody |
| 纯化方式 | ProA affinity purified |
| 性状 | Liquid |
| 缓冲液 | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| 研究背景 | Actin polymerization is required for a variety of cell functions, including chemotaxis, cell migration, cell adhesion, and platelet activation. Cells trigger actin polymerization through either the de novo nucleation of filaments from monomeric actin, the severing of existing filaments to create uncapped barbed ends, or the uncapping existing barbed ends. The nucleation of actin is a rate-limiting and unfavorable reaction in actin polymerization and therefore requires the involvement of the Arp2/3 complex, which helps create new filaments and promotes the end-to-side cross-linking of actin filaments into the branching meshwork. The Arp2/3 complex consists of the actin-related proteins Arp2 and Arp3, and various other accessory proteins. The Arp2/3 complex promotes actin nucleation by binding the pointed end of actin filaments, or by associating with the side of an existing filament, and nucleates growth in the barbed direction. In addition, the Arp2/3 complex also mediates actin cytoskeletal outgrowths that are regulated by the Rho family of small GTPases. In response to GTP-binding Cdc42, the Arp2/3 complex binds the Cdc42 substrates, namely the WASP proteins, and initiates the formation of lamellipodia and filopodia. |
| 偶联 | Unconjugated |
| 免疫原 | Recombinant Protein: C-terminal human Arp3 |
| 抗原种属 | human |
| Uniprot ID |
| 分子量 | Theoretical: 47 kDa. |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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