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Anti-Argininosuccinate synthase 1 Antibody (2S146) 是一种 Rabbit 抗体,靶向 Argininosuccinate synthase 1。Anti-Argininosuccinate synthase 1 Antibody (2S146) 可用于 WB,IHC-P,IHC-Fr,IF,IP,FCM。
Anti-Argininosuccinate synthase 1 Antibody (2S146) 是一种 Rabbit 抗体,靶向 Argininosuccinate synthase 1。Anti-Argininosuccinate synthase 1 Antibody (2S146) 可用于 WB,IHC-P,IHC-Fr,IF,IP,FCM。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 25 μL | ¥ 785 | 5日内发货 | |
| 50 μL | ¥ 1,385 | 5日内发货 | |
| 100 μL | ¥ 2,495 | 5日内发货 |
| 产品描述 | Anti-Argininosuccinate synthase 1 Antibody (2S146) is a Rabbit antibody targeting Argininosuccinate synthase 1. Anti-Argininosuccinate synthase 1 Antibody (2S146) can be used in WB,IHC-P,IHC-Fr,IF,IP,FCM. |
| Ig Type | IgG |
| 克隆号 | 2S146 |
| 反应种属 | Human,Mouse,Rat |
| 验证活性 | 1. Paraformaldehyde-fixed, paraffin embedded Mouse Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Argininosuccinate synthase 1 Monoclonal Antibody, Unconjugated (TMAB-02819) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining. 2. Paraformaldehyde-fixed, paraffin embedded Rat Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Argininosuccinate synthase 1 Monoclonal Antibody, Unconjugated (TMAB-02819) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining. 3. Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Argininosuccinate synthase 1 Monoclonal Antibody, Unconjugated (TMAB-02819) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining. 4. Paraformaldehyde-fixed, paraffin embedded Mouse Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Argininosuccinate synthase 1 Monoclonal Antibody, Unconjugated (TMAB-02819) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining. 5. Paraformaldehyde-fixed, paraffin embedded Rat Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Argininosuccinate synthase 1 Monoclonal Antibody, Unconjugated (TMAB-02819) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining. 6. Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Argininosuccinate synthase 1 Monoclonal Antibody, Unconjugated (TMAB-02819) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining. 7. Paraformaldehyde-fixed, paraffin embedded Human Ovarian Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Argininosuccinate synthase 1 Monoclonal Antibody, Unconjugated (TMAB-02819) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining. 8. 25 ug total protein per lane of various lysates (see on figure) probed with Argininosuccinate synthase 1 monoclonal antibody, unconjugated (TMAB-02819) at 1: 1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min. |
| 应用 | WBIHC-PIHC-FrIFIPFCM |
| 推荐剂量 | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; IP: 1:20-50; FCM: 1:50-100 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Rabbit |
| 构建方式 | Recombinant Antibody |
| 纯化方式 | Protein A purified |
| 性状 | Liquid |
| 缓冲液 | 10mM phosphate buffered saline (pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| 研究背景 | One of the enzymes of the urea cycle, the metabolic pathway transforming neurotoxic amonia produced by protein catabolism into inocuous urea in the liver of ureotelic animals. Catalyzes the formation of arginosuccinate from aspartate, citrulline and ATP and together with ASL it is responsible for the biosynthesis of arginine in most body tissues. |
| 免疫原 | A synthesized peptide: human Argininosuccinate synthase 1 |
| 抗原种属 | Human |
| 基因名称 | ASS1 |
| 基因ID | |
| 蛋白名称 | Argininosuccinate synthase |
| Uniprot ID |
| 分子量 | Theoretical: 47 kDa. Actual: 46 kDa. |
| 储存方式 | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |