Anti-Apolipoprotein E/APOE4 Polyclonal Antibody is a Rabbit antibody targeting Apolipoprotein E/APOE4. Anti-Apolipoprotein E/APOE4 Polyclonal Antibody can be used in IF,IHC-Fr,IHC-P,WB.

IgG

Human,Mouse,Rat

1. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Apolipoprotein E4) Polyclonal Antibody, Unconjugated (TMAB-00134) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
2. Sample:
Cerebrum (Mouse) Lysate at 40 μg
Primary: Anti-Apolipoprotein E4 (TMAB-00134) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 34 kDa
Observed band size: 32 kDa

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human APOE4

Human

APOE

Apolipoprotein E

Metabolism of lipids and lipoproteins,Lipid transport,Cholesterol Metabolism,Lipoproteins/Apolipoproteins,Endoderm,Cholesterol Metabolism,Lipid metabolism,Heart disease,Neurodegenerative disease,Alzheimer's disease,Neurodegenerative disease,Lipid metabolism,Endoderm

Mediates the binding, internalization, and catabolism of lipoprotein particles. It can serve as a ligand for the LDL (apo B/E) receptor and for the specific apo-E receptor (chylomicron remnant) of hepatic tissues.