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Anti-APLN Polyclonal Antibody 是一种 Rabbit 抗体,靶向 APLN。Anti-APLN Polyclonal Antibody 可用于 IHC-P,IHC-Fr,IF。
Anti-APLN Polyclonal Antibody 是一种 Rabbit 抗体,靶向 APLN。Anti-APLN Polyclonal Antibody 可用于 IHC-P,IHC-Fr,IF。
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
50 μL | ¥ 1,170 | 5日内发货 | |
100 μL | ¥ 1,970 | 5日内发货 | |
200 μL | ¥ 2,790 | 5日内发货 |
产品描述 | Anti-APLN Polyclonal Antibody is a Rabbit antibody targeting APLN. Anti-APLN Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF. |
疾病造模方案 | |
别名 | APJ endogenous ligand, Apelin, APEL |
Ig Type | IgG |
交叉反应 | Human,Mouse,Rat |
验证活性 | 1. Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Apelin) Polyclonal Antibody, Unconjugated (TMAB-00129) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 2. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Apelin) Polyclonal Antibody, Unconjugated (TMAB-00129) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
应用 | |
推荐剂量 | IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
抗体种类 | Polyclonal |
宿主来源 | Rabbit |
亚细胞定位 | Secreted. |
组织特异性 | Expressed in the brain with highest levels in the frontal cortex, thalamus, hypothalamus and midbrain. Secreted by the mammary gland into the colostrum and the milk. |
构建方式 | Polyclonal Antibody |
纯化方式 | Protein A purified |
性状 | Liquid |
缓冲液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
浓度 | 1 mg/mL |
研究背景 | Apelin (also known as APLN) is a peptide that in humans is encoded by the APLN gene. Apelin is the endogenous ligand for the G-protein-coupled APJ receptor that is expressed at the surface of some cell types. It is widely expressed in various organs such as the heart, lung, kidney, liver, adipose tissue, gastrointestinal tract, brain, adrenal glands, endothelium, and human plasma. |
免疫原 | KLH conjugated synthetic peptide: human Apelin |
抗原种属 | Human |
基因名称 | APLN |
基因ID | |
蛋白名称 | Apelin |
Uniprot ID | |
研究领域 | Blood Pressure regulation,Inotropics,Obesity,More Neuropeptides,GPCR |
功能 | Endogenous ligand for APJ, an alternative coreceptor with CD4 for HIV-1 infection. Inhibits HIV-1 entry in cells coexpressing CD4 and APJ. Apelin-36 has a greater inhibitory activity on HIV infection than other synthetic apelin derivatives. The oral intake in the colostrum and the milk could have a role in the modulation of the immune responses in neonates. May also have a role in the central control of body fluid homeostasis by influencing AVP release and drinking behavior. |
分子量 | Theoretical: 10 kDa. |
储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
运输方式 | Shipping with blue ice. |
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