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Anti-Apg7 Antibody (7C583)
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产品编号 TMAC-00243
别名 Ubiquitin activating enzyme E1 like protein, hAGP7, GSA7, GSA 7, DKFZp434N0735, Autophagy-related 7(yeast), Autophagy related protein 7, Autophagy 7, S. cerevisiae, homolog of, Atg7l, ATG7 autophagy related 7 homolog(S. cerevisiae), ATG7 autophagy related 7 homolog, ATG 7, APG7L, APG7, S. cerevisiae, homolog of, APG7 like, APG7 autophagy 7-like(S. cerevisiae), APG7 autophagy 7 like, Apg 7
Anti-Apg7 Antibody (7C583) 是一种 Rabbit 抗体，靶向 Apg7。Anti-Apg7 Antibody (7C583) 可用于 WB,ICC/IF,IHC,IP,FCM。
Anti-Apg7 Antibody (7C583)
Anti-Apg7 Antibody (7C583)
还可以产品编号 TMAC-00243 别名 Ubiquitin activating enzyme E1 like protein, hAGP7, GSA7, GSA 7, DKFZp434N0735, Autophagy-related 7(yeast), Autophagy related protein 7, Autophagy 7, S. cerevisiae, homolog of, Atg7l, ATG7 autophagy related 7 homolog(S. cerevisiae), ATG7 autophagy related 7 homolog, ATG 7, APG7L, APG7, S. cerevisiae, homolog of, APG7 like, APG7 autophagy 7-like(S. cerevisiae), APG7 autophagy 7 like, Apg 7
Anti-Apg7 Antibody (7C583) 是一种 Rabbit 抗体，靶向 Apg7。Anti-Apg7 Antibody (7C583) 可用于 WB,ICC/IF,IHC,IP,FCM。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,495 | 5日内发货 | |
| 100 μL | ¥ 2,485 | 5日内发货 |
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产品介绍
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偶联与修饰
抗原信息
化学信息
| 产品描述 | Anti-Apg7 Antibody (7C583) is a Rabbit antibody targeting Apg7. Anti-Apg7 Antibody (7C583) can be used in WB,ICC/IF,IHC,IP,FCM. |
| 别名 | Ubiquitin activating enzyme E1 like protein, hAGP7, GSA7, GSA 7, DKFZp434N0735, Autophagy-related 7(yeast), Autophagy related protein 7, Autophagy 7, S. cerevisiae, homolog of, Atg7l, ATG7 autophagy related 7 homolog(S. cerevisiae), ATG7 autophagy related 7 homolog, ATG 7, APG7L, APG7, S. cerevisiae, homolog of, APG7 like, APG7 autophagy 7-like(S. cerevisiae), APG7 autophagy 7 like, Apg 7 |
| Ig Type | IgG |
| 交叉反应 | Human |
| 验证活性 | 1. Western blot analysis of Apg7 on Jurkat cells lysates using anti-Apg7 antibody at 1/1,000 dilution. 2. Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Apg7 antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-Apg7 antibody. Counter stained with hematoxylin. 4. Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Apg7 antibody. Counter stained with hematoxylin. 5. Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Apg7 antibody. Counter stained with hematoxylin. 6. ICC staining Apg7 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 7. ICC staining Apg7 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 8. Flow cytometric analysis of Hela cells with Apg7 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody. |
| 应用 | |
| 推荐剂量 | WB: 1:1000-2000; IHC: 1:50-200; ICC/IF: 1:50-200; FCM: 1:50-100 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Rabbit |
| 构建方式 | Recombinant Antibody |
| 纯化方式 | ProA affinity purified |
| 性状 | Liquid |
| 缓冲液 | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| 研究背景 | In yeast, autophagy is an essential process for survival during nutrient starvation and cell differentiation. The process of autophagy is characterized as a non-selective degradation of cytoplasmic proteins into membrane stuctures called autophagosomes, and it is dependent on several proteins, including the autophagy proteins APG5 and APG7. Yeast Apg7 and the human homolog, APG7, share similarities with the ubiquitin-activating enzyme E1 in Saccharomyces cerevisiae, and are likewise responsible for enzymatically activating the autophagy conjugation system. Apg5 and the human homolog, APG5 (also designated apoptosis specific protein or APS), function as substrates for the autophagy protein APG12. These proteins are covalently bonded together to form APG12/APG5 conjugates, which are required for the progression of autophagy. |
| 偶联 | Unconjugated |
| 免疫原 | Recombinant Protein |
| Uniprot ID |
| 分子量 | Theoretical: 78 kDa. |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
计算器
比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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