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Anti-ANXA4 Antibody (3T392)

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Anti-ANXA4 Antibody (3T392)
产品编号 TMAC-00214

Anti-ANXA4 Antibody (3T392) 是一种 Rabbit 抗体,靶向 ANXA4。Anti-ANXA4 Antibody (3T392) 可用于 WB,ICC,IF,IHC,FCM。

Anti-ANXA4 Antibody (3T392)

Anti-ANXA4 Antibody (3T392)

Rating icon 还可以
Anti-ANXA4 Antibody (3T392)
产品编号 TMAC-00214

Anti-ANXA4 Antibody (3T392) 是一种 Rabbit 抗体,靶向 ANXA4。Anti-ANXA4 Antibody (3T392) 可用于 WB,ICC,IF,IHC,FCM。

规格价格库存数量
50 μL
¥ 1,490
5日内发货
100 μL
¥ 2,480
5日内发货
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产品介绍

生物活性
产品描述
Anti-ANXA4 Antibody (3T392) is a Rabbit antibody targeting ANXA4. Anti-ANXA4 Antibody (3T392) can be used in WB,ICC,IF,IHC,FCM.
Ig Type
IgG
交叉反应
Human
验证活性
1. Western blot analysis of Annexin IV on human liver tissue lysate using anti-Annexin IV antibody at 1/2,000 dilution.
2. Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Annexin IV antibody. Counter stained with hematoxylin.
3. Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Annexin IV antibody. Counter stained with hematoxylin.
4. ICC staining Annexin IV in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
5. ICC staining Annexin IV in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
6. ICC staining Annexin IV in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
7. Flow cytometric analysis of HepG2 cells with Annexin IV antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
应用
推荐剂量
WB: 1:500-2000; IHC: 1:50-200; ICC:IF: 1:50-200; FCM: 1:50-100
抗体种类
Monoclonal
宿主来源Rabbit
构建方式Recombinant Antibody
纯化方式ProA affinity purified
性状Liquid
缓冲液1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
研究背景The annexin family of calcium-binding proteins is composed of at least ten mammalian genes. It is characterized by a conserved core domain which binds to phospholipids in a Ca++-dependent manner and a unique amino terminal region which may confer binding specificity. Annexin family members have been implicated as regulators of such diverse processes as ion flux, endocytosis and exocytosis, and cellular adhesion. For example, the crystal structure of Annexin III has suggested a hydrophilic amino terminus with possible Ca++ channel activity. Similarly, Annexin V has ion channel properties. Annexin IV, also referred to as endonexin, functions to regulate Cl- flux by mediating calmodulin kinase II (CaMKII) activity and Annexin V has been shown to regulate PKC activity.
偶联与修饰
偶联
Unconjugated
抗原信息
免疫原
Recombinant Protein: human Annexin IV aa 1-200
抗原种属
human
Uniprot ID
化学信息
分子量Theoretical: 36 kDa.
存储&运输
储存方式Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
运输方式Shipping with blue ice.

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