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Anti-ANXA4 Antibody (3T392) 是一种 Rabbit 抗体,靶向 ANXA4。Anti-ANXA4 Antibody (3T392) 可用于 WB,ICC,IF,IHC,FCM。
Anti-ANXA4 Antibody (3T392) 是一种 Rabbit 抗体,靶向 ANXA4。Anti-ANXA4 Antibody (3T392) 可用于 WB,ICC,IF,IHC,FCM。
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
50 μL | ¥ 1,490 | 5日内发货 | |
100 μL | ¥ 2,480 | 5日内发货 |
产品描述 | Anti-ANXA4 Antibody (3T392) is a Rabbit antibody targeting ANXA4. Anti-ANXA4 Antibody (3T392) can be used in WB,ICC,IF,IHC,FCM. |
Ig Type | IgG |
交叉反应 | Human |
验证活性 | 1. Western blot analysis of Annexin IV on human liver tissue lysate using anti-Annexin IV antibody at 1/2,000 dilution. 2. Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Annexin IV antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Annexin IV antibody. Counter stained with hematoxylin. 4. ICC staining Annexin IV in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 5. ICC staining Annexin IV in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 6. ICC staining Annexin IV in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 7. Flow cytometric analysis of HepG2 cells with Annexin IV antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody. |
应用 | |
推荐剂量 | WB: 1:500-2000; IHC: 1:50-200; ICC:IF: 1:50-200; FCM: 1:50-100 |
抗体种类 | Monoclonal |
宿主来源 | Rabbit |
构建方式 | Recombinant Antibody |
纯化方式 | ProA affinity purified |
性状 | Liquid |
缓冲液 | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
研究背景 | The annexin family of calcium-binding proteins is composed of at least ten mammalian genes. It is characterized by a conserved core domain which binds to phospholipids in a Ca++-dependent manner and a unique amino terminal region which may confer binding specificity. Annexin family members have been implicated as regulators of such diverse processes as ion flux, endocytosis and exocytosis, and cellular adhesion. For example, the crystal structure of Annexin III has suggested a hydrophilic amino terminus with possible Ca++ channel activity. Similarly, Annexin V has ion channel properties. Annexin IV, also referred to as endonexin, functions to regulate Cl- flux by mediating calmodulin kinase II (CaMKII) activity and Annexin V has been shown to regulate PKC activity. |
偶联 | Unconjugated |
免疫原 | Recombinant Protein: human Annexin IV aa 1-200 |
抗原种属 | human |
Uniprot ID |
分子量 | Theoretical: 36 kDa. |
储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
运输方式 | Shipping with blue ice. |
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