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Anti-AMPK β1 Antibody (7G100) 是一种 Rabbit 抗体,靶向 AMPK β1。Anti-AMPK β1 Antibody (7G100) 可用于 WB,FCM。
Anti-AMPK β1 Antibody (7G100) 是一种 Rabbit 抗体,靶向 AMPK β1。Anti-AMPK β1 Antibody (7G100) 可用于 WB,FCM。
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
50 μL | ¥ 1,485 | 5日内发货 | |
100 μL | ¥ 2,490 | 5日内发货 |
产品描述 | Anti-AMPK β1 Antibody (7G100) is a Rabbit antibody targeting AMPK β1. Anti-AMPK β1 Antibody (7G100) can be used in WB,FCM. |
别名 | protein kinase, AMP-activated, noncatalytic, beta-1, protein kinase, AMP-activated, beta 1 non-catalytic subunit, Protein kinase AMP activated non catalytic subunit beta 1, PRKAB1, MGC17785, HAMPKb, E430008F22, AU021155, AMPKβ1, AMPK-BETA-1, AMPKbeta1, AMPKb1, AMPKb, AMPK subunit beta-1, AMPK beta1, AMPK beta 1 chain, AMPK b1, AMPK, AMP-activated, noncatalytic, beta-1, AMP-ACTIVATED PROTEIN KINASE, NONCATALYTIC, BETA-1, AMP-activated protein kinase beta subunit, AAKB1_HUMAN, 5''-AMP-activated protein kinase subunit beta-1, 5'-AMP-activated protein kinase beta-1 subunit, 1300015D22Rik |
Ig Type | IgG |
交叉反应 | Human,Mouse,Rat |
验证活性 | 1. Western blot analysis of AMPK beta 1 on A431 cells lysates using anti-AMPK beta 1 antibody at 1/1,000 dilution. 2. Flow cytometric analysis of A431 cells with AMPK beta 1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody |
应用 | |
推荐剂量 | WB: 1:1000-2000; FCM: 1:50-100 |
抗体种类 | Monoclonal |
宿主来源 | Rabbit |
构建方式 | Recombinant Antibody |
纯化方式 | ProA affinity purified |
性状 | Liquid |
缓冲液 | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
研究背景 | AMPK (for 5'-AMP-activated protein kinase) is a heterotrimeric complex com-prising a catalytic α subunit and regulatory β and γ subunits. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming bio-synthetic pathways. AMPK is activated by high AMP and low ATP through a mechanism involving allosteric regulation, promotion of phosphorylation by an upstream protein kinase known as AMPK kinase, and inhibition of dephosphorylation. Activated AMPK can phosphorylate and regulate in vivo hydroxy-methylglutaryl-CoA reductase and acetyl-CoA carboxylase, which are key regulatory enzymes of sterol synthesis and fatty acid synthesis, respectively. The human AMPKα1 and AMPKα2 genes encode 548 amino acid and 552 amino acid proteins, respectively. Human AMPKβ1 encodes a 271 amino acid protein and human AMPKβ2 encodes a 272 amino acid protein. The human AMPKγ1 gene encodes a 331 amino acid protein. Human AMPKγ2 and AMPKγ3, which are 569 and 492 amino acid proteins, respectively, contain unique N-terminal domains and may participate directly in the binding of AMP within the AMPK complex. |
偶联 | Unconjugated |
免疫原 | Recombinant Protein |
Uniprot ID |
分子量 | Theoretical: 38 kDa. |
储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
运输方式 | Shipping with blue ice. |
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