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顶部生物活性储存和溶解信息计算器

Anti-alpha Actinin 4 Antibody (7D211)

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产品编号 TMAC-00154

别名 αActinin 4, α Actinin 4, Non-muscle alpha-actinin 4, Non muscle alpha actinin 4, FSGS1, FSGS 1, FSGS, Focal segmental glomerulosclerosis 1, F-Actin Cross-Linking Protein, F actin cross linking protein, DKFZp686K23158, Alpha-actinin-4, alphaActinin 4, ACTN4_HUMAN, ACTN4, ACTN 4, actinin4, Actinin alpha 4, actinin 4, aActinin 4, a Actinin 4

Anti-alpha Actinin 4 Antibody (7D211) 是一种 Rabbit 抗体，靶向 alpha Actinin 4。Anti-alpha Actinin 4 Antibody (7D211) 可用于 FCM,ICC,IF,IHC,IP,WB。

Anti-alpha Actinin 4 Antibody (7D211)

Anti-alpha Actinin 4 Antibody (7D211)

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产品编号 TMAC-00154 别名 αActinin 4, α Actinin 4, Non-muscle alpha-actinin 4, Non muscle alpha actinin 4, FSGS1, FSGS 1, FSGS, Focal segmental glomerulosclerosis 1, F-Actin Cross-Linking Protein, F actin cross linking protein, DKFZp686K23158, Alpha-actinin-4, alphaActinin 4, ACTN4_HUMAN, ACTN4, ACTN 4, actinin4, Actinin alpha 4, actinin 4, aActinin 4, a Actinin 4

Anti-alpha Actinin 4 Antibody (7D211) 是一种 Rabbit 抗体，靶向 alpha Actinin 4。Anti-alpha Actinin 4 Antibody (7D211) 可用于 FCM,ICC,IF,IHC,IP,WB。

规格价格库存数量
50 μL
¥ 1,485
5日内发货
100 μL
¥ 2,495
5日内发货
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产品介绍

生物活性
产品描述
Anti-alpha Actinin 4 Antibody (7D211) is a Rabbit antibody targeting alpha Actinin 4. Anti-alpha Actinin 4 Antibody (7D211) can be used in FCM,ICC,IF,IHC,IP,WB.
别名αActinin 4, α Actinin 4, Non-muscle alpha-actinin 4, Non muscle alpha actinin 4, FSGS1, FSGS 1, FSGS, Focal segmental glomerulosclerosis 1, F-Actin Cross-Linking Protein, F actin cross linking protein, DKFZp686K23158, Alpha-actinin-4, alphaActinin 4, ACTN4_HUMAN, ACTN4, ACTN 4, actinin4, Actinin alpha 4, actinin 4, aActinin 4, a Actinin 4
Ig Type
IgG
克隆号
7D211
交叉反应
Human,Mouse,Rat
验证活性
1. Western blot analysis of alpha Actinin 4 on different lysates using anti-alpha Actinin 4 antibody at 1/500 dilution.Positive control: Lane 1: Hela, Lane 2: PC-12, Lane 3: NIH-3T3, Lane 4: Rat liver tissue, Lane 5: A431, Lane 6: HepG2.
2. Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-alpha Actinin 4 antibody. Counter stained with hematoxylin.
3. Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-alpha Actinin 4 antibody. Counter stained with hematoxylin.
4. ICC staining alpha Actinin 4 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
5. ICC staining alpha Actinin 4 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
6. ICC staining alpha Actinin 4 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
7. Flow cytometric analysis of A549 cells with alpha Actinin 4 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).
verifiedActivityverifiedActivityverifiedActivityverifiedActivityverifiedActivityverifiedActivityverifiedActivity
应用FCMICCIFIHCIPWB
推荐剂量
WB: 1:500-2000; IHC: 1:50-200; ICC: 1:100-500; IP: 1:10-50; FCM: 1:50-100
抗体种类
Monoclonal
宿主来源Rabbit
构建方式Recombinant Antibody
纯化方式ProA affinity purified
性状Liquid
缓冲液1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
研究背景The spectrin gene family encodes a diverse group of cytoskeletal proteins that include spectrins, dystrophins and α-actinins. There are four tissue-specific α-actinins, namely α-actinin-1, α-actinin-2, α-actinin-3 and α-actinin-4, which are localized to muscle and non-muscle cells, including skeletal, cardiac and smooth muscle cells, as well as within the cytoskeleton. Each α-actinin protein contains one Actin-binding domain, two calponin-homology domains, two EF-hand domains and four spectrin repeats, through which they function as bundling proteins that can cross-link F-Actin, thus anchoring Actin to a variety of intracellular structures. Defects in the gene encoding α-actinin-4 are the cause of focal segmental glomerulosclerosis 1 (FSGS1), a common renal lesion characterized by decreasing kidney function and, ultimately, renal failure. are actually sensitive to the Profilin proteins in these foods.
偶联与修饰
偶联
Unconjugated
抗原信息
免疫原
Recombinant Protein
Uniprot ID
O43707
化学信息
分子量Theoretical: 105 kDa.
存储&运输
储存方式Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
运输方式Shipping with blue ice.

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