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Anti-AIF Antibody (9K13)
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产品编号 TMAC-00102
别名 Programmed Cell Death Protein 8, PDCD8, Apoptosis-Inducing Factor 1 Mitochondrial, AIFM1, AIF
Anti-AIF Antibody (9K13) 是一种 Rabbit 抗体，靶向 AIF。Anti-AIF Antibody (9K13) 可用于 WB,ICC,IHC,IP,FCM。
Anti-AIF Antibody (9K13)
Anti-AIF Antibody (9K13)
还可以产品编号 TMAC-00102 别名 Programmed Cell Death Protein 8, PDCD8, Apoptosis-Inducing Factor 1 Mitochondrial, AIFM1, AIF
Anti-AIF Antibody (9K13) 是一种 Rabbit 抗体，靶向 AIF。Anti-AIF Antibody (9K13) 可用于 WB,ICC,IHC,IP,FCM。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,490 | 5日内发货 | |
| 100 μL | ¥ 2,480 | 5日内发货 |
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产品介绍
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化学信息
| 产品描述 | Anti-AIF Antibody (9K13) is a Rabbit antibody targeting AIF. Anti-AIF Antibody (9K13) can be used in WB,ICC,IHC,IP,FCM. |
| 别名 | Programmed Cell Death Protein 8, PDCD8, Apoptosis-Inducing Factor 1 Mitochondrial, AIFM1, AIF |
| Ig Type | IgG |
| 交叉反应 | Human,Mouse,Rat |
| 验证活性 | 1. Western blot analysis of AIF on different lysates using anti-AIF antibody at 1/1,000 dilution. Positive control: Lane 1: Hela, Lane 2: SKOV-3. 2. Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-AIF antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-AIF antibody. Counter stained with hematoxylin. 4. Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-AIF antibody. Counter stained with hematoxylin. 5. Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-AIF antibody. Counter stained with hematoxylin. 6. ICC staining AIF in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 7. ICC staining AIF in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 8. Flow cytometric analysis of Hela cells with AIF antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody. |
| 应用 | |
| 推荐剂量 | WB: 1:1000-5000; IHC: 1:50-200; ICC: 1:50-200; FCM: 1:50-100 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Rabbit |
| 构建方式 | Recombinant Antibody |
| 纯化方式 | ProA affinity purified |
| 性状 | Liquid |
| 缓冲液 | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| 研究背景 | A key event in the apoptotic process is the opening of the mitochondrial permeability transition pore, an event that is regulated by Bcl-2 family proteins, resulting in the release of several proteins from the mitochondrial intermembrane space. Several of these proteins participate in apoptosis, including cytochrome c, procaspases 2, 3, and 9, and AIF (apoptosis-inducing factor). AIF has been shown to cause DNA fragmentation and chromatin condensation and to induce the release of cytochrome c and caspase-9 from mitochondria. Bcl-2 overexpression has been shown to prevent the release of AIF from mitochondria, but not to block its apoptogenic activity. |
| 偶联 | Unconjugated |
| 免疫原 | Recombinant Protein |
| Uniprot ID |
| 分子量 | Theoretical: 67 kDa. |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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