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Anti-AFP Antibody (6V105) 是一种 Mouse 抗体,靶向 AFP。Anti-AFP Antibody (6V105) 可用于 WB,IHC,IF。
Anti-AFP Antibody (6V105) 是一种 Mouse 抗体,靶向 AFP。Anti-AFP Antibody (6V105) 可用于 WB,IHC,IF。
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
50 μL | ¥ 1,330 | 5日内发货 | |
100 μL | ¥ 2,180 | 5日内发货 |
产品描述 | Anti-AFP Antibody (6V105) is a Mouse antibody targeting AFP. Anti-AFP Antibody (6V105) can be used in WB,IHC,IF. |
别名 | α-fetoprotein, α-fetoglobulin, α-1-fetoprotein, Alpha-fetoprotein, Alpha-fetoglobulin, Alpha-1-fetoprotein, Afp |
交叉反应 | Human |
验证活性 | 1. Western blot analysis of HepG2, diluted at 1:2,000. 2. Immunohistochemical analysis of paraffin-embedded Human-breast-cancer tissue. 1,AFP alpha 1 Fetoprotein Monoclonal Antibody(17C5) was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only. 3. Immunohistochemical analysis of paraffin-embedded Human gallbladder. 1, Antibody was diluted at 1:100(4°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min). 4. Immunofluorescence analysis of Human-breast-cancer tissue. 1,AFP alpha 1 Fetoprotein Monoclonal Antibody(17C5)(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B. |
应用 | |
推荐剂量 | WB: 1:2000; IF: 1:200; IHC: 1:50-300 |
抗体种类 | Monoclonal |
宿主来源 | Mouse |
构建方式 | Hybridoma Monoclonal Antibody |
纯化方式 | The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen. |
性状 | Liquid |
缓冲液 | PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol. |
研究背景 | This gene encodes alpha-fetoprotein, a major plasma protein produced by the yolk sac and the liver during fetal life. Alpha-fetoprotein expression in adults is often associated with hepatoma or teratoma. However, hereditary persistance of alpha-fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the alpha-fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. Alpha-fetoprotein is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of alpha-fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly. [provided by RefSeq, Jul 2008], |
偶联 | Unconjugated |
免疫原 | A synthesized peptide: AFP alpha 1 Fetoprotein |
基因ID | |
Uniprot ID |
分子量 | Theoretical: 70 kDa. |
储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
运输方式 | Shipping with blue ice. |
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