还可以
Anti-ADRB1 Polyclonal Antibody 2 是一种 Rabbit 抗体，靶向 ADRB1。Anti-ADRB1 Polyclonal Antibody 2 可用于 WB,IHC-P,IHC-Fr,IF,FCM。
还可以Anti-ADRB1 Polyclonal Antibody 2 是一种 Rabbit 抗体，靶向 ADRB1。Anti-ADRB1 Polyclonal Antibody 2 可用于 WB,IHC-P,IHC-Fr,IF,FCM。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,175 | 5日内发货 | |
| 100 μL | ¥ 1,975 | 5日内发货 | |
| 200 μL | ¥ 2,780 | 5日内发货 |
| 产品描述 | Anti-ADRB1 Polyclonal Antibody 2 is a Rabbit antibody targeting ADRB1. Anti-ADRB1 Polyclonal Antibody 2 can be used in WB,IHC-P,IHC-Fr,IF,FCM. |
| 别名 | RHR, BETA1AR, beta 1 adrenoreceptor, beta 1 adrenoceptor, beta 1 Adrenergic Receptor, B1AR, Adrenoceptor beta 1, Adrenergic beta 1 receptor, ADRB1R, ADRB1, ADRB 1, ADR B1 |
| Ig Type | IgG |
| 交叉反应 | Human,Mouse,Rat (predicted:Dog,Pig) |
| 验证活性 | 1. Blank control: U937. Primary Antibody (green line): Rabbit Anti-ADRB1 antibody (TMAB-00069) Dilution: 1 μg/10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-AF647 Dilution: 1 μg/test. Protocol The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. 2. Sample: Lane 1: Stomach (Mouse) Lysate at 40 μg Lane 2: Heart (Mouse) Lysate at 40 μg Lane 3: Heart (Rat) Lysate at 40 μg Primary: Anti-ADRB1 (TMAB-00069) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 52 kDa Observed band size: 50 kDa 3. Paraformaldehyde-fixed, paraffin embedded (human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (ADRB1) Polyclonal Antibody, Unconjugated (TMAB-00069) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining. |
| 应用 | |
| 推荐剂量 | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 1ug/Test |
| 抗体种类 | Polyclonal |
| 宿主来源 | Rabbit |
| 亚细胞定位 | Cell membrane; Multi-pass membrane protein. Note=Localized at the plasma membrane. Found in the Golgi upon GOPC overexpression. |
| 构建方式 | Hybridoma Polyclonal Antibody |
| 纯化方式 | Protein A purified |
| 性状 | Liquid |
| 缓冲液 | Preservative: 0.02% Proclin300, Constituents: 1% BSA, 0.01M PBS, pH7.4. |
| 研究背景 | Beta-adrenergic receptors (Beta-1 adrenoreceptor) mediate the catecholamine-induced activation of adenylate cyclase through the action of G proteins. This receptor binds epinephrine and norepinephrine with approximately equal affinity. Subcellular location cell membrane; Multi-pass membrane protein. Homologous desensitization of the receptor is mediated by its phosphorylation by beta-adrenergic receptor kinase. Belongs to the G-protein coupled receptor 1 family. |
| 免疫原 | KLH conjugated synthetic peptide: human ADRB1 |
| 抗原种属 | Human |
| 基因ID | |
| Uniprot ID | |
| 研究领域 | GPCR,Adrenalin,Adrenergic Receptors,Blood Pressure regulation |
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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