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Anti-acnB Antibody-HRP (6F295)
还可以产品编号 TMAY-02484H
Anti-acnB Antibody-HRP (6F295) 是一种 HRP-conjugated Mouse 抗体，靶向 acnB。Anti-acnB Antibody-HRP (6F295) 可用于 ELISA。
Anti-acnB Antibody-HRP (6F295)
Anti-acnB Antibody-HRP (6F295)
还可以产品编号 TMAY-02484H
Anti-acnB Antibody-HRP (6F295) 是一种 HRP-conjugated Mouse 抗体，靶向 acnB。Anti-acnB Antibody-HRP (6F295) 可用于 ELISA。
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产品介绍
生物活性
偶联与修饰
抗原信息
| 产品描述 | Anti-acnB Antibody-HRP (6F295) is a HRP-conjugated Mouse antibody targeting acnB. Anti-acnB Antibody-HRP (6F295) can be used in ELISA. |
| Ig Type | Monoclonal Mouse IgG2a |
| 克隆号 | 6F295 |
| 交叉反应 | E. coli |
| 应用 | ELISA |
| 推荐剂量 | ELISA: 0.1-1 μg/ml |
| 抗体种类 | Monoclonal |
| 宿主来源 | Mouse |
| 构建方式 | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Escherichia coli acnB ( TMPY-02375; P36683; Met1-Val865). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography and conjugated with horseradish-peroxidase (HRP). |
| 纯化方式 | Protein A |
| 性状 | Liquid |
| 缓冲液 | 0.2 μm filtered solution in PBS |
| 研究背景 | Escherichia coli contains two major aconitases (Acns), AcnA and AcnB. They are distantly related monomeric Fe-S proteins that contain different arrangements of four structural domains. acnA is specifically subject to SoxRS-mediated activation, whereas acnB encodes the major aconitase that is synthesized earlier in the growth cycle than AcnA. It is concluded that AcnB is the major citric acid cycle enzyme. Aconitate hydratase 2 (acnB) catalyzes the isomerization of citrate to isocitrate via cis-aconitate as well as the dehydration of 2-methylisocitrate to cis-2-methylaconitate, thus it functions as the major citric-acid-cycle enzyme during exponential growth. Escherichia coli acnB serves as either an enzymic catalyst or a mRNA-binding post-transcriptional regulator, depending on the status of its iron sulfur cluster. AcnB represents a large, distinct group of Gram-negative bacterial aconitases that have an altered domain organization relative to mitochondrial aconitase and other aconitases. |
| 偶联 | HRP |
| 其他可选修饰符 | Unconjugated |
| 可提供抗体种类 | 2 |
| 免疫原 | Recombinant Escherichia coli acnB (TMPY-02375) |
| 抗原种属 | E. coli |
存储&运输
| 储存方式 | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. Keep away from direct sunlight. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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