Anti-Acetyl-Histone H4 (Lys12) Antibody (8Z904) is a Mouse antibody targeting Acetyl-Histone H4 (Lys12). Anti-Acetyl-Histone H4 (Lys12) Antibody (8Z904) can be used in ICC/IF, IF, IHC-Fr, IHC-P, WB.

IgG

8Z904

Human (predicted:Mouse,Rat)

1. Tissue: Human neuroblastoma
Section type: Formalin fixed & Paraffin-embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH9.0 Primary dilution: 1:200
Primary incubation condition: 1 hour at room temperature
Secondary: SP Kit (Mouse)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal for TMAB-00036
2. Cell line: HeLa
Fixative: 4% Paraformaldehyde
Permeabilization: 0.1% TritonX-100
Primary dilution: 1:100
Primary incubation condition: 4°C overnight
Secondary: Goat Anti-Mouse IgG
Nuclear counter stain: DAPI (Blue)
Counter stain: Tubulin (Red)
Comment: Color green is the positive signal for TMAB-00036
3. Blocking buffer: 5% NFDM/TBST
Primary dilution: 1:2000
Primary incubation condition: 2 hours at room temperature
Secondary: Goat Anti-Mouse IgG H&L (HRP)
Lysate: (-) HeLa, (+) HeLa+Sodium butyrate (30mM, 4hr)
Protein loading quantity: 20 μg
Exposure time: 60 s
Predicted MW: 11 kDa
Observed MW: 11 kDa

ICC/IF=1:50-100; IF=1:100-500; IHC-Fr=1:100-500; IHC-P=1:100-500; WB=1:500-2000

Monoclonal

H4C1

Histone H4

Acetylated,ChIP antibodies

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.