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Anti-AARSD1 Antibody (3K421) 是一种 Rabbit 抗体,靶向 AARSD1。Anti-AARSD1 Antibody (3K421) 可用于 WB,ELISA,ICC/IF。
Anti-AARSD1 Antibody (3K421) 是一种 Rabbit 抗体,靶向 AARSD1。Anti-AARSD1 Antibody (3K421) 可用于 WB,ELISA,ICC/IF。
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
100 μL | ¥ 2,294 | 5日内发货 |
产品描述 | Anti-AARSD1 Antibody (3K421) is a Rabbit antibody targeting AARSD1. Anti-AARSD1 Antibody (3K421) can be used in WB,ELISA,ICC/IF. |
别名 | MGC2744, AlaXp, AARSD 1 |
Ig Type | Rabbit IgG |
克隆号 | 3K421 |
交叉反应 | Human |
验证活性 | 1. Immunofluorescence staining of Human AARSD1 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human AARSD1 monoclonal antibody (1:60) at 4°C overnight. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.
2. Anti-AARSD1 rabbit monoclonal antibody at 1:500 dilution. -Lane A: Hela Whole Cell Lysate. -Lane B: A549 Whole Cell lysate. -Lysates/proteins at 30 μg per lane. -Secondary -Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. -Developed using the Odyssey technique. -Performed under reducing conditions. -Predicted band size:45 kDa. -Observed band size:50 kDa |
应用 | WB,ELISA,ICC/IF |
推荐剂量 | WB: 1:500-1:2000; ELISA: 1:5000-1:10000; ICC-IF: 1:20-1:100 |
抗体种类 | Monoclonal |
宿主来源 | Rabbit |
构建方式 | This antibody was obtained from a rabbit immunized with purified, recombinant Human AARSD1 (Catalog #14280-H07E; Q9BTE6-2; Thr176-Glu525). |
纯化方式 | Protein A |
性状 | Liquid |
缓冲液 | 0.2 μm filtered solution in PBS |
研究背景 | AARSD1 belongs to the class-II aminoacyl-tRNA synthetase family, Alax-L subfamily. AARSD1 binds 1 zinc ion per subunit functions in trans to edit the amino acid moiety from incorrectly charged tRNA(Ala). Four transcript variants have been described for AARSD1: NM_25267.3, NM_113642.2, NM_1142653.1 and NM_1142654.1. It has been determined that the latter two variants represent a distinct upstream locus, which is now represented by GeneID:1885848 (PTGES3L), while the former two variants represent readthrough transcripts between PTGES3L and this locus (AARSD1). The readthrough locus (PTGES3L-AARSD1) is now represented by GeneID:188585. |
偶联 | Unconjugated |
免疫原 | Recombinant Protein: Human AARSD1 Protein |
抗原种属 | Human |
储存方式 | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
运输方式 | Shipping with blue ice. |
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