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Anti-A-Raf Antibody (2P32) 是一种 Rabbit 抗体,靶向 A-Raf。Anti-A-Raf Antibody (2P32) 可用于 WB,ICC/IF,IHC,FCM。
Anti-A-Raf Antibody (2P32) 是一种 Rabbit 抗体,靶向 A-Raf。Anti-A-Raf Antibody (2P32) 可用于 WB,ICC/IF,IHC,FCM。
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
50 μL | ¥ 1,480 | 5日内发货 | |
100 μL | ¥ 2,490 | 5日内发货 |
产品描述 | Anti-A-Raf Antibody (2P32) is a Rabbit antibody targeting A-Raf. Anti-A-Raf Antibody (2P32) can be used in WB,ICC/IF,IHC,FCM. |
别名 | v raf oncogene homolog 1 (murine sarcoma 3611 virus), v raf murine sarcoma 3611 viral oncogene homolog 1, v raf murine sarcoma 3611 viral oncogene homolog, Serine/threonine-protein kinase A-Raf, Ras binding protein DA Raf, RAFA1, RAFA 1, Proto-oncogene Pks, Proto-oncogene A-Raf-1, Proto-oncogene A-Raf, Proto oncogene Pks, PKS2, PKS 2, PKS, Oncogene PKS2, Oncogene Araf1, ARAF1, ARAF_HUMAN, ARaf proto oncogene serine/threonine protein kinase, ARAF 1, ARAF, A Raf proto oncogene serine/threonine protein kinase, A raf 1 |
Ig Type | IgG |
交叉反应 | Human |
验证活性 | 1. Western blot analysis of A-RAF on Hela cells lysates using anti-A-RAF antibody at 1/1,000 dilution. 2. Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-A-RAF antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-A-RAF antibody. Counter stained with hematoxylin. 4. ICC staining A-RAF in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 5. ICC staining A-RAF in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 6. ICC staining A-RAF in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 7. Flow cytometric analysis of Hela cells with A-RAF antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody. |
应用 | |
推荐剂量 | WB: 1:1000-2000; IHC: 1:50-200; ICC/IF: 1:50-200; FCM: 1:50-100 |
抗体种类 | Monoclonal |
宿主来源 | Rabbit |
构建方式 | Recombinant Antibody |
纯化方式 | ProA affinity purified |
性状 | Liquid |
缓冲液 | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
研究背景 | Several serine/threonine protein kinases have been implicated as intermediates in signal transduction pathways. These include ERK/MAP kinases, ribosomal S6 kinase (Rsk) and Raf-1. Raf-1 is a cytoplasmic protein with intrinsic serine/ threonine activity. It is broadly expressed in nearly all cell lines tested to date and is the cellular homolog of v-Raf, the product of the transforming gene of the 3611 strain of murine sarcoma virus. The unregulated kinase activity of the v-Raf protein has been associated with transformation and mitogenesis while the activity of Raf-1 is normally suppressed by a regulatory N-terminal domain. A-Raf, a second member of the Raf gene family of serine/threonine protein kinases, exhibits substantial homology to Raf-1 within the kinase domain of the two molecules, but less homology elsewhere. Expression of A-Raf is found at highest levels in urogenital tissues and kidney and at lowest level in brain tissue. |
偶联 | Unconjugated |
免疫原 | Recombinant Protein |
Uniprot ID |
分子量 | Theoretical: 68 kDa. |
储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
运输方式 | Shipping with blue ice. |
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