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Anti-A-Raf Antibody (2P32)

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产品编号 TMAC-00261

别名 v raf oncogene homolog 1 (murine sarcoma 3611 virus), v raf murine sarcoma 3611 viral oncogene homolog 1, v raf murine sarcoma 3611 viral oncogene homolog, Serine/threonine-protein kinase A-Raf, Ras binding protein DA Raf, RAFA1, RAFA 1, Proto-oncogene Pks, Proto-oncogene A-Raf-1, Proto-oncogene A-Raf, Proto oncogene Pks, PKS2, PKS 2, PKS, Oncogene PKS2, Oncogene Araf1, ARAF1, ARAF_HUMAN, ARaf proto oncogene serine/threonine protein kinase, ARAF 1, ARAF, A Raf proto oncogene serine/threonine protein kinase, A raf 1

Anti-A-Raf Antibody (2P32) 是一种 Rabbit 抗体，靶向 A-Raf。Anti-A-Raf Antibody (2P32) 可用于 FCM,ICC/IF,IHC,WB。

Anti-A-Raf Antibody (2P32)

Anti-A-Raf Antibody (2P32)

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产品编号 TMAC-00261 别名 v raf oncogene homolog 1 (murine sarcoma 3611 virus), v raf murine sarcoma 3611 viral oncogene homolog 1, v raf murine sarcoma 3611 viral oncogene homolog, Serine/threonine-protein kinase A-Raf, Ras binding protein DA Raf, RAFA1, RAFA 1, Proto-oncogene Pks, Proto-oncogene A-Raf-1, Proto-oncogene A-Raf, Proto oncogene Pks, PKS2, PKS 2, PKS, Oncogene PKS2, Oncogene Araf1, ARAF1, ARAF_HUMAN, ARaf proto oncogene serine/threonine protein kinase, ARAF 1, ARAF, A Raf proto oncogene serine/threonine protein kinase, A raf 1

Anti-A-Raf Antibody (2P32) 是一种 Rabbit 抗体，靶向 A-Raf。Anti-A-Raf Antibody (2P32) 可用于 FCM,ICC/IF,IHC,WB。

规格价格库存数量
50 μL
¥ 1,480
5日内发货
100 μL
¥ 2,490
5日内发货
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产品介绍

生物活性
产品描述
Anti-A-Raf Antibody (2P32) is a Rabbit antibody targeting A-Raf. Anti-A-Raf Antibody (2P32) can be used in FCM,ICC/IF,IHC,WB.
别名v raf oncogene homolog 1 (murine sarcoma 3611 virus), v raf murine sarcoma 3611 viral oncogene homolog 1, v raf murine sarcoma 3611 viral oncogene homolog, Serine/threonine-protein kinase A-Raf, Ras binding protein DA Raf, RAFA1, RAFA 1, Proto-oncogene Pks, Proto-oncogene A-Raf-1, Proto-oncogene A-Raf, Proto oncogene Pks, PKS2, PKS 2, PKS, Oncogene PKS2, Oncogene Araf1, ARAF1, ARAF_HUMAN, ARaf proto oncogene serine/threonine protein kinase, ARAF 1, ARAF, A Raf proto oncogene serine/threonine protein kinase, A raf 1
Ig Type
IgG
克隆号
2P32
交叉反应
Human
验证活性
1. Western blot analysis of A-RAF on Hela cells lysates using anti-A-RAF antibody at 1/1,000 dilution.
2. Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-A-RAF antibody. Counter stained with hematoxylin.
3. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-A-RAF antibody. Counter stained with hematoxylin.
4. ICC staining A-RAF in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
5. ICC staining A-RAF in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
6. ICC staining A-RAF in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
7. Flow cytometric analysis of Hela cells with A-RAF antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
verifiedActivityverifiedActivityverifiedActivityverifiedActivityverifiedActivityverifiedActivityverifiedActivity
应用FCMICC/IFIHCWB
推荐剂量
WB: 1:1000-2000; IHC: 1:50-200; ICC/IF: 1:50-200; FCM: 1:50-100
抗体种类
Monoclonal
宿主来源Rabbit
构建方式Recombinant Antibody
纯化方式ProA affinity purified
性状Liquid
缓冲液1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
研究背景Several serine/threonine protein kinases have been implicated as intermediates in signal transduction pathways. These include ERK/MAP kinases, ribosomal S6 kinase (Rsk) and Raf-1. Raf-1 is a cytoplasmic protein with intrinsic serine/ threonine activity. It is broadly expressed in nearly all cell lines tested to date and is the cellular homolog of v-Raf, the product of the transforming gene of the 3611 strain of murine sarcoma virus. The unregulated kinase activity of the v-Raf protein has been associated with transformation and mitogenesis while the activity of Raf-1 is normally suppressed by a regulatory N-terminal domain. A-Raf, a second member of the Raf gene family of serine/threonine protein kinases, exhibits substantial homology to Raf-1 within the kinase domain of the two molecules, but less homology elsewhere. Expression of A-Raf is found at highest levels in urogenital tissues and kidney and at lowest level in brain tissue.
偶联与修饰
偶联
Unconjugated
抗原信息
免疫原
Recombinant Protein
Uniprot ID
P10398
化学信息
分子量Theoretical: 68 kDa.
存储&运输
储存方式Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
运输方式Shipping with blue ice.

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