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Anti-4-1BB Ligand/TNFSF9 Antibody (8F465)
还可以产品编号 TMAY-03070
Anti-4-1BB Ligand/TNFSF9 Antibody (8F465) 是一种 Rabbit 抗体，靶向 4-1BB Ligand/TNFSF9。Anti-4-1BB Ligand/TNFSF9 Antibody (8F465) 可用于 ELISA,FCM,ICC/IF。
Anti-4-1BB Ligand/TNFSF9 Antibody (8F465)
Anti-4-1BB Ligand/TNFSF9 Antibody (8F465)
还可以产品编号 TMAY-03070
Anti-4-1BB Ligand/TNFSF9 Antibody (8F465) 是一种 Rabbit 抗体，靶向 4-1BB Ligand/TNFSF9。Anti-4-1BB Ligand/TNFSF9 Antibody (8F465) 可用于 ELISA,FCM,ICC/IF。
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产品介绍
生物活性
偶联与修饰
抗原信息
| 产品描述 | Anti-4-1BB Ligand/TNFSF9 Antibody (8F465) is a Rabbit antibody targeting 4-1BB Ligand/TNFSF9. Anti-4-1BB Ligand/TNFSF9 Antibody (8F465) can be used in ELISA,FCM,ICC/IF. |
| Ig Type | Monoclonal Rabbit IgG |
| 克隆号 | 8F465 |
| 交叉反应 | Mouse |
| 验证活性 | 1. Confocal immunofluorescence analysis of Mouse TNFSF9 in RAW264.7 cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with Rabbit anti-Mouse TNFSF9 monoclonal antibody (1:100) at 4°C overnight. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to plasma membrane. 2. Flow cytometric analysis of Mouse TNFSF9 expression on Raw264.7 cells. Cells were stained with purified anti-Mouse TNFSF9, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells. |
| 应用 | ELISA,FCM,ICC/IF |
| 推荐剂量 | ELISA: 1:1000-1:2000; ICC-IF: 1:50-1:1000; FCM: 1:25-1:100 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Rabbit |
| 构建方式 | This antibody was obtained from a rabbit immunized with purified, recombinant Mouse TNFSF9 / 4-1BBL (rM TNFSF9; TMPY-00959; Arg 104-Glu 309; NP_033430.1). |
| 纯化方式 | Protein A |
| 性状 | Liquid |
| 缓冲液 | 0.2 μm filtered solution in PBS |
| 研究背景 | 4-1BB ligand is the high affinity ligand of 4-1BB, also known as CD137L or TNFSF9. It is shown to be a type II surface glycoprotein belonging to the TNF superfamily. Expression of 4-1BBL is restricted to APCs, such as dendritic cells, macrophages, and activated B cells. Members of the TNF-TNF receptor superfamily have been shown to play critical roles in regulating cellular activation, differentiation and apoptosis. Several studies have reported that 4-1BBL/4-1BB interaction provided a co-stimulatory signal to T cells, and increased T cell proliferation and cytokines production. In addition, 4-1BBL is involved in cancers, infectious diseases and autoimmune diseases.Cancer ImmunotherapyCo-stimulatory Immune Checkpoint TargetsImmune CheckpointImmune Checkpoint Detection: AntibodiesImmune Checkpoint Detection: ELISA AntibodiesImmune Checkpoint Detection: FCM AntibodiesImmune Checkpoint Detection: ICC AntibodiesImmune Checkpoint TargetsImmunotherapyTargeted Therapy |
| 偶联 | Unconjugated |
| 其他可选修饰符 | APC/PerCP/FITC/PE |
| 可提供抗体种类 | 5 |
| 免疫原 | Recombinant Mouse TNFSF9 protein (TMPY-00959) |
| 抗原种属 | Mouse |
| 研究领域 | Cancer Drug Targets |
存储&运输
| 储存方式 | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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