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顶部生物活性储存和溶解信息计算器

Anti-14‑3‑3 theta/YWHAQ Antibody (5O606)

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Anti-14‑3‑3 theta/YWHAQ Antibody (5O606)
产品编号 TMAC-00008
别名 tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, θ, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, theta, HS1, 41701, 1C5

Anti-14‑3‑3 theta/YWHAQ Antibody (5O606) 是一种 Rabbit 抗体，靶向 14‑3‑3 theta/YWHAQ。Anti-14‑3‑3 theta/YWHAQ Antibody (5O606) 可用于 WB,ICC/IF,FCM。

Anti-14‑3‑3 theta/YWHAQ Antibody (5O606)

Anti-14‑3‑3 theta/YWHAQ Antibody (5O606)

Rating icon 还可以
Anti-14‑3‑3 theta/YWHAQ Antibody (5O606)
产品编号 TMAC-00008 别名 tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, θ, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, theta, HS1, 41701, 1C5

Anti-14‑3‑3 theta/YWHAQ Antibody (5O606) 是一种 Rabbit 抗体，靶向 14‑3‑3 theta/YWHAQ。Anti-14‑3‑3 theta/YWHAQ Antibody (5O606) 可用于 WB,ICC/IF,FCM。

规格价格库存数量
50 μL
¥ 1,495
5日内发货
100 μL
¥ 2,485
5日内发货
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产品介绍

生物活性
产品描述
Anti-14‑3‑3 theta/YWHAQ Antibody (5O606) is a Rabbit antibody targeting 14‑3‑3 theta/YWHAQ. Anti-14‑3‑3 theta/YWHAQ Antibody (5O606) can be used in WB,ICC/IF,FCM.
别名tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, θ, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, theta, HS1, 41701, 1C5
Ig Type
IgG
交叉反应
Human,Mouse,Rat
验证活性
1. Western blot analysis of 14-3-3 Theta on different lysates using anti-14-3-3 Theta antibody at 1/1,000 dilution. Positive control: Lane 1: Hela, Lane 2: MCF-7.
2. ICC staining 14-3-3 Theta in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
3. ICC staining 14-3-3 Theta in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
4. ICC staining 14-3-3 Theta in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
5. Flow cytometric analysis of SH-SY-5Y cells with 14-3-3 Theta antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
应用
WB
ICC/IF
FCM
推荐剂量
WB: 1:1000-5000; ICC/IF: 1:100-500; FCM: 1:50-100
抗体种类
Monoclonal
宿主来源Rabbit
构建方式Recombinant Antibody
纯化方式ProA affinity purified
性状Liquid
缓冲液1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
研究背景14-3-3 proteins regulate many cellular processes relevant to cancer biology, notably apoptosis, mitogenic signaling and cell-cycle checkpoints. Seven isoforms comprise this family of signaling intermediates, denoted 14-3-3 β, γ, ε, ζ, η, θ and ??. 14-3-3 proteins form dimers that present two binding sites for ligand proteins, thereby bringing together two proteins that may not otherwise associate. These ligands largely share a 14-3-3 consensus binding motif and exhibit serine/threonine phosphorylation. 14-3-3 proteins function in broad regulation of these ligand proteins, by cytoplasmic sequestration, occupation of interaction domains and import/export sequences, prevention of degradation, activation/repression of enzymatic activity and facilitation of protein modification, and thus loss of expression contributes to a vast array of pathogenic cellular activities.
偶联与修饰
偶联
Unconjugated
抗原信息
免疫原
Recombinant Protein
Uniprot ID
P27348
化学信息
分子量Theoretical: 28 kDa.
存储&运输
储存方式Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
运输方式Shipping with blue ice.

计算器

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关键词

HS-1HS 1

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