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Anti-14-3-3 eta/YWHAH Antibody (5D580) 是一种 Rabbit 抗体,靶向 14-3-3 eta/YWHAH。Anti-14-3-3 eta/YWHAH Antibody (5D580) 可用于 WB,FCM。
Anti-14-3-3 eta/YWHAH Antibody (5D580) 是一种 Rabbit 抗体,靶向 14-3-3 eta/YWHAH。Anti-14-3-3 eta/YWHAH Antibody (5D580) 可用于 WB,FCM。
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
50 μL | ¥ 1,485 | 5日内发货 | |
100 μL | ¥ 2,485 | 5日内发货 |
产品描述 | Anti-14-3-3 eta/YWHAH Antibody (5D580) is a Rabbit antibody targeting 14-3-3 eta/YWHAH. Anti-14-3-3 eta/YWHAH Antibody (5D580) can be used in WB,FCM. |
别名 | YWHA1, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, eta |
Ig Type | IgG |
交叉反应 | Human,Mouse,Rat |
验证活性 | 1. Western blot analysis of 14-3-3 gamma on different lysates using anti-14-3-3 gamma antibody at 1/1,000 dilution. Positive control: Lane 1: 293T, Lane 2: A431 Lane 3: Hela. 2. Flow cytometric analysis of K562 cells with 14-3-3 gamma antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody. |
应用 | |
推荐剂量 | WB: 1:1000-2000; FCM: 1:10-50 |
抗体种类 | Monoclonal |
宿主来源 | Rabbit |
构建方式 | Recombinant Antibody |
纯化方式 | ProA affinity purified |
性状 | Liquid |
缓冲液 | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
研究背景 | 14-3-3 proteins regulate many cellular processes relevant to cancer biology, notably apoptosis, mitogenic signaling and cell-cycle checkpoints. Seven isoforms comprise this family of signaling intermediates, denoted 14-3-3 b, g, e, z, h, q and s. 14-3-3 proteins form dimers that present two binding sites for ligand proteins, thereby bringing together two proteins that may not otherwise associate. These ligands largely share a 14-3-3 consensus binding motif and exhibit serine/threonine phosphorylation. 14-3-3 proteins function in broad regulation of these ligand proteins; by cytoplasmic sequestration, occupation of interaction domains and import/export sequences, prevention of degradation, activation/repression of enzymatic activity, and facilitation of protein modification. Loss of expression contributes to a vast array of pathogenic cellular activities. |
偶联 | Unconjugated |
免疫原 | Recombinant Protein |
Uniprot ID |
分子量 | Theoretical: 28 kDa. |
储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
运输方式 | Shipping with blue ice. |
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