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Anti-14-3-3 beta Antibody (9I180)
还可以产品编号 TMAY-01251
Anti-14-3-3 beta Antibody (9I180) 是一种 Mouse 抗体，靶向 14-3-3 beta。Anti-14-3-3 beta Antibody (9I180) 可用于 ELISA,FCM,ICC/IF。
Anti-14-3-3 beta Antibody (9I180)
Anti-14-3-3 beta Antibody (9I180)
还可以产品编号 TMAY-01251
Anti-14-3-3 beta Antibody (9I180) 是一种 Mouse 抗体，靶向 14-3-3 beta。Anti-14-3-3 beta Antibody (9I180) 可用于 ELISA,FCM,ICC/IF。
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产品介绍
生物活性
偶联与修饰
抗原信息
| 产品描述 | Anti-14-3-3 beta Antibody (9I180) is a Mouse antibody targeting 14-3-3 beta. Anti-14-3-3 beta Antibody (9I180) can be used in ELISA,FCM,ICC/IF. |
| Ig Type | Monoclonal Mouse IgG2b |
| 克隆号 | 9I180 |
| 交叉反应 | Human |
| 验证活性 | 1. Immunofluorescence staining of Human YWHAB in MCF7 cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with Mouse anti-Human YWHAB monoclonal antibody (1:60) at 4°C overnight. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-mouse IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm. 2. Flow cytometric analysis of Human YWHAB expression on Molt-4 cells. The cells were treated according to manufacturer’s manual (BD Pharmingen™ Cat. No. 554714), stained with purified anti-Human YWHAB, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells. |
| 应用 | ELISA,FCM,ICC/IF |
| 推荐剂量 | ELISA: 1:1000-1:2000; ICC-IF: 1:20-1:100; FCM: 1:25-1:100 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Mouse |
| 构建方式 | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human 14-3-3 beta / YWHAB (rh 14-3-3 beta / YWHAB; TMPY-01266; NP_003395.1; Met1-Asn246). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. |
| 纯化方式 | Protein A |
| 性状 | Liquid |
| 缓冲液 | 0.2 μm filtered solution in PBS |
| 研究背景 | 14-3-3 beta / YWHAB is a member of the 14-3-3 proteins family. 14-3-3 proteins are a group of highly conserved proteins that are involved in many vital cellular processes such as metabolism, protein trafficking, signal transduction, apoptosis and cell cycle regulation. 14-3-3 proteins are mainly localized in the synapses and neuronal cytoplasm, and seven isoforms have been identified in mammals. This family of proteins was initially identified as adaptor proteins which bind to phosphoserine-containing motifs. Binding motifs and potential functions of 14-3-3 proteins are now recognized to have a wide range of functional relevance. 14-3-3 beta / YWHAB is found in both plants and mammals, and this protein is 100% identical to the mouse ortholog. 14-3-3 beta / YWHAB interacts with CDC25 phosphatases, RAF1 and IRS1 proteins, suggesting its role in diverse biochemical activities related to signal transduction, such as cell division and regulation of insulin sensitivity. 14-3-3 beta / YWHAB has also been implicated in the pathogenesis of small cell lung cancer. 14-3-3 beta / YWHAB binding negatively regulates RSK1 activity to maintain signal specificity and that association/dissociation of the 14-3-3beta-RSK1 complex is likely to be important for mitogen-mediated RSK1 activation. |
| 偶联 | Unconjugated |
| 其他可选修饰符 | APC/PerCP/FITC/PE |
| 可提供抗体种类 | 5 |
| 免疫原 | Recombinant Human 14-3-3 beta / YWHAB protein (TMPY-01266) |
| 抗原种属 | Human |
| 研究领域 | phospho-Serine/phospho-Threonine Binding Proteins, Adaptor Proteins, Apoptosis Adaptor Proteins |
存储&运输
| 储存方式 | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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