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Anti-14-3-3 beta/alpha Antibody (6Z701)
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产品编号 TMAC-00001
别名 YWHAA, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, β, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta, KCIP-1, HS1, HEL-S-1, GW128, 14-3-3 β
Anti-14-3-3 beta/alpha Antibody (6Z701) 是一种 Rabbit 抗体，靶向 14-3-3 beta/alpha。Anti-14-3-3 beta/alpha Antibody (6Z701) 可用于 WB,ICC,IHC,IP,FCM。
Anti-14-3-3 beta/alpha Antibody (6Z701)
Anti-14-3-3 beta/alpha Antibody (6Z701)
还可以产品编号 TMAC-00001 别名 YWHAA, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, β, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta, KCIP-1, HS1, HEL-S-1, GW128, 14-3-3 β
Anti-14-3-3 beta/alpha Antibody (6Z701) 是一种 Rabbit 抗体，靶向 14-3-3 beta/alpha。Anti-14-3-3 beta/alpha Antibody (6Z701) 可用于 WB,ICC,IHC,IP,FCM。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,490 | 5日内发货 | |
| 100 μL | ¥ 2,495 | 5日内发货 |
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偶联与修饰
抗原信息
化学信息
| 产品描述 | Anti-14-3-3 beta/alpha Antibody (6Z701) is a Rabbit antibody targeting 14-3-3 beta/alpha. Anti-14-3-3 beta/alpha Antibody (6Z701) can be used in WB,ICC,IHC,IP,FCM. |
| 别名 | YWHAA, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, β, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta, KCIP-1, HS1, HEL-S-1, GW128, 14-3-3 β |
| Ig Type | IgG |
| 交叉反应 | Human,Mouse,Rat |
| 验证活性 | 1. Western blot analysis of 14-3-3 alpha+beta on different lysates using anti-14-3-3 alpha/beta antibody at 1/1,000 dilution. Positive control: Lane 1: Hela, Lane 2: 293T, Lane 3: HepG2. 2. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-14-3-3 alpha+beta antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-14-3-3 alpha+beta antibody. Counter stained with hematoxylin. 4. Immunohistochemical analysis of paraffin-embedded mouse skin tissue using anti-14-3-3 alpha+beta antibody. Counter stained with hematoxylin. 5. Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-14-3-3 alpha+beta antibody. Counter stained with hematoxylin. 6. ICC staining 14-3-3 alpha+beta in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 7. ICC staining 14-3-3 alpha+beta in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 8. ICC staining 14-3-3 alpha+beta in SHG-44 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 9. Flow cytometric analysis of Hela cells with 14-3-3 alpha+beta antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody. |
| 应用 | |
| 推荐剂量 | WB: 1:1000-5000; IHC: 1:50-200; ICC: 1:100-500; FCM: 1:50-100 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Rabbit |
| 构建方式 | Recombinant Antibody |
| 纯化方式 | ProA affinity purified |
| 性状 | Liquid |
| 缓冲液 | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| 研究背景 | Members of the 14-3-3 family of proteins are highly conserved proteins, localized in neurons, and are axonally transported to the nerve terminals. They are also present, at lower levels, in various other eukaryotic tissues. 14-3-3 proteins appear to play important roles in a variety of signal transduction pathways, including those involved in cell cycle regulation and cell survival. Because 14-3-3 proteins bind to specific phosphoserine-containing sequences they are likely to have an important role in signaling pathways mediated by serine/threonine protein kinases. Evidence indicates 14-3-3 is required for Raf 1 kinase activity and phosphorylation among many other functions. |
| 偶联 | Unconjugated |
| 免疫原 | Recombinant Protein |
| Uniprot ID |
| 分子量 | Theoretical: 28 kDa. |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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