Acetylated-BSA is a well-characterized polypeptide with strong antigenic properties. It can elicit a significant immune response, thus validating the accuracy and reliability of experimental methods. Ac-BSA serves as a positive control in ELISA or WB experiments, particularly in assays involving acetylated lysine monoclonal or polyclonal antibodies. Additionally, it enhances encapsulation efficiency at low PLGA concentrations, with PLGA being a biocompatible, degradable, and controlled-release polymer used in drug delivery.