Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMOG (Dimethyloxalylglycine) 是 α-酮戊二酸辅因子的拮抗剂,是一种 HIF 脯氨酰羟化酶抑制剂,可导致蛋白HIF-1α的积聚和稳定,诱导细胞自噬。它是一种促血管生成剂,在结肠炎和腹泻动物模型中起保护作用。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
50 mg | ¥ 541 | 现货 | ||
100 mg | ¥ 837 | 现货 | ||
200 mg | ¥ 1,458 | 现货 | ||
500 mg | ¥ 3,262 | 现货 |
产品描述 | DMOG (Dimethyloxalylglycine), an antagonist of the α-ketoglutarate cofactor, is an inhibitor for HIF prolyl hydroxylase. |
体外活性 | DMOG shows only weakly active in the microsomal system, but efficiently suppresses hydroxyproline synthesis in intact cells. [1] DMOG reduces FGF-2-induced proliferation and cyclin A expression by inhibiting prolyl hydroxylase activity in HPASMC. [3] |
体内活性 | DMOG inhibits endogenous HIF inactivation and induces angiogenesis in the ischaemic skeletal muscles of mice. [2] Up-regulation of hypoxia-inducible factor-1α by DMOG may be the cardioprotective mechanism of ischemic postconditioning in hyperlipidemic rats[4]. |
细胞实验 | To analyze DNA synthesis as an index of cellular proliferation, VSMC are plated in 48-well plates (5,000 per square centimeter) in growth medium, incubated overnight, and serum-deprived (1% FCS) for 24 h. Replicate wells are then stored at −70°C for baseline (day 0) cell counts, and fresh medium with or without growth factors is added to the remaining wells, which are incubated 72-96 h in 20 or 5% O2. Days 0 and 3 or 4 cell counts are determined by lysing cells in a buffer containing a fluorescent dye, which has minimal fluorescence by itself but fluoresces when bound to DNA or RNA. Absolute cell numbers are calculated by comparing the fluorescence of specimens with that of a standard curve similarly prepared using a known number of cells. (Only for Reference) |
别名 | Dimethyloxalylglycine, Dimethyloxallyl Glycine, Dimethyloxaloylglycine, 二甲基草酰甘氨酸 |
分子量 | 175.14 |
分子式 | C6H9NO5 |
CAS No. | 89464-63-1 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
H2O: 17.54mg/mL(100 mM), Sonication is recommended.
DMSO: 17.5 mg/mL(100 mM)
Ethanol: 35 mg/mL (199.84 mM)
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
H2O / DMSO / Ethanol | 1 mM | 5.7097 mL | 28.5486 mL | 57.0972 mL | 142.7429 mL |
5 mM | 1.1419 mL | 5.7097 mL | 11.4194 mL | 28.5486 mL | |
10 mM | 0.571 mL | 2.8549 mL | 5.7097 mL | 14.2743 mL | |
20 mM | 0.2855 mL | 1.4274 mL | 2.8549 mL | 7.1371 mL | |
50 mM | 0.1142 mL | 0.571 mL | 1.1419 mL | 2.8549 mL | |
100 mM | 0.0571 mL | 0.2855 mL | 0.571 mL | 1.4274 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
DMOG 89464-63-1 Angiogenesis Autophagy Chromatin/Epigenetic Metabolism HIF/HIF Prolyl-Hydroxylase HIF Dimethyloxalylglycine Dimethyloxallyl Glycine diarrhoea Hypoxia-inducible factors stabilisation Inhibitor accmulation HIFs inhibit HIF-PH pro-angiogenic Dimethyloxaloylglycine 二甲基草酰甘氨酸 inhibitor