SH3PXD2A 重组蛋白, Human (His & Myc)

SH3PXD2A Protein, Human, Recombinant (His & Myc)

产品编号 TMPH-02106

Adapter protein involved in invadopodia and podosome formation, extracellular matrix degradation and invasiveness of some cancer cells. Binds matrix metalloproteinases (ADAMs), NADPH oxidases (NOXs) and phosphoinositides. Acts as an organizer protein that allows NOX1- or NOX3-dependent reactive oxygen species (ROS) generation and ROS localization. In association with ADAM12, mediates the neurotoxic effect of amyloid-beta peptide.

SH3PXD2A Protein, Human, Recombinant (His & Myc)

产品编号 TMPH-02106

规格	价格	库存
20 μg	¥ 1,800	20日内发货
100 μg	¥ 3,760	20日内发货
1 mg	¥ 16,000	20日内发货

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SH3PXD2A Protein, Human, Recombinant (His & Myc)产品问题解答

问请问实验使用正常鼠，PEG300和吐温80这些会对小鼠有影响吗？

答PEG300 具有适度的粘稠度，有利于药物的溶解和给药过程，同时也能够提供良好的耐受性，不会对动物造成不良影响。PEG400 可以替代 PEG300。<br/>Tween-80已被用作评估实验药物和毒物的行为影响的工具，且没有明显的副作用。

常见问题解答

问产品的溶解度是多少？

答建议您先查询TargetMol官网、陶术官网，看该抑制剂的产品介绍，找到溶解度信息。如果没有相应信息，建议联系技术对该化合物的溶解度进行测定。

问抑制剂是否需要灭菌？

答如果您使用 DMSO 配制：不建议灭菌，DMSO 本身具有极强的杀菌力，配制好的溶液就是无菌溶液。如果您确实担心的，可以放置 4℃ 冰箱过夜放置即可；依然有顾虑的话，需用有机系专用滤膜过滤。如果您使用水配制：可以用 0.22 μm 滤膜过滤灭菌。

问抑制剂母液的储存方式？

答母液配置完成后，一般储存于 -80℃，可以存 1 年以上。建议多分装几管，避免反复冻融，常用的存于 4 度，可放一周以上。

问细胞实验中需要加多少抑制剂？

答通常建议您参考同模型实验发表的文献报道；除参考文献报道以外，还需要通过预实验做“剂量-效应曲线”确定最佳作用浓度（浓度梯度）；通过“时间-效应曲线”确定最佳孵育时间（处理时间梯度）。另外，抑制剂的使用量受多种因素影响，包括抑制对象的可接触性、细胞通透性、孵育时间、细胞种类等等。我们建议通过检索文献确定使用抑制剂的起始浓度。如果有报道的 Ki 值或 IC50 值，可以采用其 5-10 倍的量开始尝试以达到抑制酶活性的最佳效果。如果抑制剂的 Ki 值或 IC50 值未知，则需要在更广泛的范围尝试抑制剂的使用浓度，并采用 Michaelis-Menten 动力学计算 Ki 值。一般设立溶解抑制剂时所采用的溶剂作为对照，以排除溶剂的非特异性影响。

问母液配置的计算方法？

答（1） c=n/v=m/M/v 浓度=物质的量/体积=质量/相对分子质量/体积（2）官网产品详情页下方有溶液配制表格，可以参考计算好的加入溶剂体积

产品信息

生物活性	Activity has not been tested. It is theoretically active, but we cannot guarantee it. If you require protein activity, we recommend choosing the eukaryotic expression version first.
产品描述	Adapter protein involved in invadopodia and podosome formation, extracellular matrix degradation and invasiveness of some cancer cells. Binds matrix metalloproteinases (ADAMs), NADPH oxidases (NOXs) and phosphoinositides. Acts as an organizer protein that allows NOX1- or NOX3-dependent reactive oxygen species (ROS) generation and ROS localization. In association with ADAM12, mediates the neurotoxic effect of amyloid-beta peptide.
种属	Human
表达系统	E. coli
标签	N-10xHis, C-Myc
蛋白编号	Q5TCZ1
别名	Tyrosine kinase substrate with five SH3 domains,TKS5,SH3PXD2A,SH3MD1,SH3 multiple domains protein 1,SH3 and PX domain-containing protein 2A,KIAA0418,Five SH3 domain-containing protein,FISH,Adapter protein TKS5
氨基酸序列	PDPSGKELDTVPAKGRQNEGKSDSLEKIERRVQALNTVNQSKKATPPIPSKPPGGFGKTSGTPAVKMRNGVRQVAVRPQSVFVSP
蛋白构建	902-986 aa
蛋白纯度	> 90% as determined by SDS-PAGE.
分子量	14.0 kDa (predicted)
内毒素	< 1.0 EU/μg of the protein as determined by the LAL method.
缓冲液	Tris-based buffer, 50% glycerol
复溶方法	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
存储	Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
运输方式	In general, Lyophilized powders are shipping with blue ice. Solutions are shipping with dry ice.
研究背景	Adapter protein involved in invadopodia and podosome formation, extracellular matrix degradation and invasiveness of some cancer cells. Binds matrix metalloproteinases (ADAMs), NADPH oxidases (NOXs) and phosphoinositides. Acts as an organizer protein that allows NOX1- or NOX3-dependent reactive oxygen species (ROS) generation and ROS localization. In association with ADAM12, mediates the neurotoxic effect of amyloid-beta peptide.

SH3PXD2A Protein, Human, Recombinant (His & Myc)

SH3PXD2A Protein, Human, Recombinant (His & Myc)

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