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CD28 Protein, Human/Cynomolgus/Rhesus macaque, Recombinant (His & Avi)

产品编号 TMPK-00110CD28 Protein, Human/Cynomolgus/Rhesus macaque, Recombinant (His & Avi)

CD28 is the receptor for CD80 (B7-1) and CD86 (B7-2) proteins.When activated by Toll-like receptor ligands, the CD80 expression is upregulated in antigen presenting cells (APCs). The CD86 expression on antigen presenting cells is constitutive. CD28 is the only B7 receptor constitutively expressed on naive T cells. CD28 Protein, Human/Cynomolgus/Rhesus macaque, Recombinant (His & Avi) is expressed in HEK293 mammalian cells with N-His-Avi tag. The predicted molecular weight is 18.2 kDa and the accession number is P10747-1.

CD28 Protein, Human/Cynomolgus/Rhesus macaque, Recombinant (His & Avi)

CD28 Protein, Human/Cynomolgus/Rhesus macaque, Recombinant (His & Avi)

产品编号 TMPK-00110CD28 Protein, Human/Cynomolgus/Rhesus macaque, Recombinant (His & Avi)
CD28 is the receptor for CD80 (B7-1) and CD86 (B7-2) proteins.When activated by Toll-like receptor ligands, the CD80 expression is upregulated in antigen presenting cells (APCs). The CD86 expression on antigen presenting cells is constitutive. CD28 is the only B7 receptor constitutively expressed on naive T cells. CD28 Protein, Human/Cynomolgus/Rhesus macaque, Recombinant (His & Avi) is expressed in HEK293 mammalian cells with N-His-Avi tag. The predicted molecular weight is 18.2 kDa and the accession number is P10747-1.
规格价格库存数量
100 μg
¥ 2,960
5日内发货
500 μg
¥ 11,800
5日内发货
1 mg
¥ 19,700
5日内发货
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实验操作小课堂
常见问题解答
溶解度中写的“< 1 mg/mL refers to the slightly soluble or insoluble ”是什么意思?
这句话指的是,如果溶解度 < 1 mg/mL,那我们就认为它是微溶或不溶的,这是针对写的“DMSO:Insoluble”或“DMSO:slightly soluble”这类化合物的。
超声久了会不会对化合物的结构有影响?
建议以较低频率对化合物进行超声,不会对化合物的结构造成影响。
如何稀释工作液?
根据您的工作液浓度,计算母液所需要稀释的倍数和所需母液的体积。建议用水、生理盐水、PBS等溶剂进行稀释,如果是细胞实验,可以使用培养基。 取适当的溶剂缓慢加入到母液中,直到达到您的工作液浓度,可以通过涡旋或者移液枪轻轻吹打帮助混匀。 我们的化合物大部分是脂溶性的,所以在使用 PBS 或者培养基等稀释工作液的时候,会有沉淀析出,这些都是正常现象,大多时候可以通过超声完全溶解。 工作液建议现配现用!
母液配置的计算方法?
(1) c=n/v=m/M/v 浓度=物质的量/体积=质量/相对分子质量/体积 (2) 官网产品详情页下方有溶液配制表格,可以参考计算好的加入溶剂体积
准备进行动物实验,收到大包装粉末后,如何分装溶解?
根据配制出来的溶液是不是澄清的确定一次性能配多少:如果配制出来是澄清溶液,可以一次性多配制一些,储存于4度冰箱,大概一周配一次,放久了可能会失效;如果配制出来是混悬液,建议每次使用的时候都现配现用。
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产品信息

生物活性
1. Immobilized Human/Cynomolgus/Rhesus macaque CD28, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-CD28 Antibody, hFc Tag with the EC50 of 18.4ng/ml determined by ELISA (QC Test). 2. Anti-CD28 antibody captured on Protein A chip, can bind Human/Cynomolgus/Rhesus macaque CD28, His Tag with an affinity constant of 5.15nM as determined in a SPR assay.
产品描述
CD28 is the receptor for CD80 (B7-1) and CD86 (B7-2) proteins.When activated by Toll-like receptor ligands, the CD80 expression is upregulated in antigen presenting cells (APCs). The CD86 expression on antigen presenting cells is constitutive. CD28 is the only B7 receptor constitutively expressed on naive T cells. CD28 Protein, Human/Cynomolgus/Rhesus macaque, Recombinant (His & Avi) is expressed in HEK293 mammalian cells with N-His-Avi tag. The predicted molecular weight is 18.2 kDa and the accession number is P10747-1.
种属
Human,Cynomolgus,Rhesus
表达系统
HEK293 Cells
标签N-His-Avi
蛋白编号P10747-1
别名
MGC138290,CD28 molecule,CD28 antigen (Tp44),CD28
蛋白构建
Asn19-Pro152
蛋白纯度
> 95% as determined by Tris-Bis PAGE
分子量18.2 kDa (predicted). Due to glycosylation, the protein migrates to 36-52 kDa based on Tris-Bis PAGE result.
内毒素< 1 EU/μg by the LAL method.
缓冲液Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization.
复溶方法
Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
存储
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
运输方式In general, Lyophilized powders are shipping with blue ice.
研究背景
CD28 is the receptor for CD80 (B7-1) and CD86 (B7-2) proteins.When activated by Toll-like receptor ligands, the CD80 expression is upregulated in antigen presenting cells (APCs). The CD86 expression on antigen presenting cells is constitutive. CD28 is the only B7 receptor constitutively expressed on naive T cells.

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