CD28 is the receptor for CD80 (B7-1) and CD86 (B7-2) proteins.When activated by Toll-like receptor ligands, the CD80 expression is upregulated in antigen presenting cells (APCs). The CD86 expression on antigen presenting cells is constitutive. CD28 is the only B7 receptor constitutively expressed on naive T cells. CD28 Protein, Human/Cynomolgus/Rhesus macaque, Recombinant (His & Avi) is expressed in HEK293 mammalian cells with N-His-Avi tag. The predicted molecular weight is 18.2 kDa and the accession number is P10747-1.
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
100 μg | ¥ 2,960 | 5日内发货 | ||
500 μg | ¥ 11,800 | 5日内发货 | ||
1 mg | ¥ 19,700 | 5日内发货 |
生物活性 | 1. Immobilized Human/Cynomolgus/Rhesus macaque CD28, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-CD28 Antibody, hFc Tag with the EC50 of 18.4ng/ml determined by ELISA (QC Test). 2. Anti-CD28 antibody captured on Protein A chip, can bind Human/Cynomolgus/Rhesus macaque CD28, His Tag with an affinity constant of 5.15nM as determined in a SPR assay. |
产品描述 | CD28 is the receptor for CD80 (B7-1) and CD86 (B7-2) proteins.When activated by Toll-like receptor ligands, the CD80 expression is upregulated in antigen presenting cells (APCs). The CD86 expression on antigen presenting cells is constitutive. CD28 is the only B7 receptor constitutively expressed on naive T cells. CD28 Protein, Human/Cynomolgus/Rhesus macaque, Recombinant (His & Avi) is expressed in HEK293 mammalian cells with N-His-Avi tag. The predicted molecular weight is 18.2 kDa and the accession number is P10747-1. |
种属 | Human,Cynomolgus,Rhesus |
表达系统 | HEK293 Cells |
标签 | N-His-Avi |
蛋白编号 | P10747-1 |
别名 | CD28 molecule, MGC138290, CD28, CD28 antigen (Tp44) |
蛋白构建 | Asn19-Pro152 |
蛋白纯度 | > 95% as determined by Tris-Bis PAGE |
分子量 | 18.2 kDa (predicted). Due to glycosylation, the protein migrates to 36-52 kDa based on Tris-Bis PAGE result. |
内毒素 | < 1 EU/μg by the LAL method. |
缓冲液 | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization. |
复溶方法 | Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing. |
存储 |
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
运输方式 |
In general, Lyophilized powders are shipping with blue ice. |
研究背景 | CD28 is the receptor for CD80 (B7-1) and CD86 (B7-2) proteins.When activated by Toll-like receptor ligands, the CD80 expression is upregulated in antigen presenting cells (APCs). The CD86 expression on antigen presenting cells is constitutive. CD28 is the only B7 receptor constitutively expressed on naive T cells. |
bottom
您可能有的问题的答案可以在重组蛋白操作手册中找到
CD28 Protein, Human/Cynomolgus/Rhesus macaque, Recombinant (His & Avi) CD28 molecule MGC-138290 MGC 138290 Tp44 MGC138290 CD28 antigen CD-28 CD28 CD28 antigen (Tp44) CD 28 recombinant recombinant-proteins proteins protein