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Anti-TAP-Tag Antibody (3J975) 是一种 Mouse 抗体,靶向 TAP-Tag。Anti-TAP-Tag Antibody (3J975) 可用于 ELISA, FCM, ICC/IF, IF, IHC-Fr, IHC-P, WB。
Anti-TAP-Tag Antibody (3J975) 是一种 Mouse 抗体,靶向 TAP-Tag。Anti-TAP-Tag Antibody (3J975) 可用于 ELISA, FCM, ICC/IF, IF, IHC-Fr, IHC-P, WB。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 200 μg | ¥ 5,585 | 5日内发货 | |
| 100 μL | ¥ 965 | 5日内发货 | |
| 500 μL | ¥ 3,995 | 5日内发货 |
| 产品描述 | Anti-TAP-Tag Antibody (3J975) is a Mouse antibody targeting TAP-Tag. Anti-TAP-Tag Antibody (3J975) can be used in ELISA, FCM, ICC/IF, IF, IHC-Fr, IHC-P, WB. |
| Ig Type | IgG |
| 克隆号 | 3J975 |
| 反应种属 | Species independent |
| 应用 | ELISAFCMICC/IFIFIHC-FrIHC-PWB |
| 推荐剂量 | ELISA=1:1000-5000; FCM=1 μg/Test; ICC/IF=1:100-500; IF=1:200-1000; IHC-Fr=1:200-1000; IHC-P=1:200-1000; WB=1:1000-5000 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Mouse |
| 构建方式 | Hybridoma Monoclonal Antibody |
| 纯化方式 | Protein G purified |
| 性状 | Liquid |
| 缓冲液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 浓度 | 1mg/ml |
| 研究背景 | Tandem affinity purification (TAP) is a purification technique for studying protein–protein interactions. It involves creating a fusion protein with a designed piece, the TAP tag, on the end. The original TAP method involves the fusion of the TAP tag to the C-terminus of the protein under study. The TAP tag consists of calmodulin binding peptide (CBP) from the N-terminal, followed by tobacco etch virus protease (TEV protease) cleavage site and Protein A, which binds tightly to IgG. The relative order of the modules of the tag is important because Protein A needs to be at the extreme end of the fusion protein so that the entire complex can be retrieved using an IgG matrix. |
| 免疫原 | Recombinant Protein: TAP-Tag |
| 储存方式 | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
| 存储 | store at low temperature | -20°C for 1 year |