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Anti-NSDHL Antibody (1Q116)

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产品编号 TMAH-00828
别名 Sterol-4-alpha-carboxylate 3-dehydrogenase, Protein H105e3, H105E3, EC 1.1.1.170, decarboxylating

Anti-NSDHL Antibody (1Q116) 是一种抗体,靶向 NSDHL。Anti-NSDHL Antibody (1Q116) 可用于 ELISA, WB, IHC。

Anti-NSDHL Antibody (1Q116)

Anti-NSDHL Antibody (1Q116)

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产品编号 TMAH-00828 别名 Sterol-4-alpha-carboxylate 3-dehydrogenase, Protein H105e3, H105E3, EC 1.1.1.170, decarboxylating

Anti-NSDHL Antibody (1Q116) 是一种抗体,靶向 NSDHL。Anti-NSDHL Antibody (1Q116) 可用于 ELISA, WB, IHC。

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50 μL
¥ 1,315
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100 μL
¥ 2,180
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产品介绍

生物活性
产品描述
Anti-NSDHL Antibody (1Q116) is an antibody targeting NSDHL. Anti-NSDHL Antibody (1Q116) can be used in ELISA, WB, IHC.
别名Sterol-4-alpha-carboxylate 3-dehydrogenase, Protein H105e3, H105E3, EC 1.1.1.170, decarboxylating
Ig Type
Rabbit IgG
克隆号
1Q116
交叉反应
Human
验证活性
1. Western Blot
-Positive WB detected in: HepG2 whole cell lysate, Hela whole cell lysate, PC-3 whole cell lysate, HEK293 whole cell lysate
-All lanes: NSDHL antibody at 1:1000
-Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution
-Predicted band size: 42 kDa
-Observed band size: 42 kDa
2. IHC image of TMAH-00828 diluted at 1:100 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
应用
ELISA, WB, IHC
推荐剂量
WB:1:500-1:2000; IHC:1:50-1:200.
抗体种类
Monoclonal
亚细胞定位Endoplasmic reticulum membrane; Single-pass membrane protein. Lipid droplet.
构建方式Recombinant Antibody
纯化方式Affinity-chromatography
性状Liquid
缓冲液Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
研究背景Catalyzes the NAD(P)(+)-dependent oxidative decarboxylation of the C4 methyl groups of 4-alpha-carboxysterols in post-squalene cholesterol biosynthesis. Plays also a role in the regulation of the endocytic trafficking of EGFR.
偶联与修饰
偶联
Unconjugated
抗原信息
免疫原
A synthetic peptide: Human NSDHL
抗原种属
Human
基因ID
50814
Uniprot ID
研究领域
Cancer, Cardiovascular, Metabolism, Signal transduction
存储&运输
储存方式Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
运输方式Shipping with blue ice.

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