Powder: -20°C for 3 years | In solvent: -80°C for 1 year
UCF 101 是竞争性的促凋亡蛋白酶Omi/HtrA2选择性抑制剂。它对 MI/R 损伤具有明显的心脏保护作用,还具有一定的神经保护作用。它在 543 nm 处具有天然的红色荧光,可用于监视其进入哺乳动物细胞的能力。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
1 mg | ¥ 348 | 现货 | ||
2 mg | ¥ 491 | 现货 | ||
5 mg | ¥ 798 | 现货 | ||
10 mg | ¥ 1,390 | 现货 | ||
25 mg | ¥ 2,890 | 现货 | ||
50 mg | ¥ 4,280 | 现货 | ||
100 mg | ¥ 5,970 | 现货 | ||
1 mL * 10 mM (in DMSO) | ¥ 898 | 现货 |
产品描述 | UCF 101 is a specific inhibitor of HtrA2 and reduces apoptosis in PC12 cells. |
靶点活性 | His-Omi:9.5 μM (IC50) |
体外活性 | PC12 cells treated with 60 μM 6-OHDA for 24 h had significantly decreased cell viability compared to that of controls. A low concentration (2.5 μM) of UCF 101 decreased the apoptosis rate of the PD cell model, but a high concentration (≥10 μM) increased the apoptosis rate, compared to that of controls. 6-OHDA upregulated the expression of HtrA2, α-syn, CHOP, Grp78 and active caspase-3 and reduced the levels of TH and XIAP[3]. |
体内活性 | Administration of UCF 101 shortly before reperfusion reduced postischemic myocardial apoptosis in a dose-dependent fashion, with the maximal protective effect observed at a dose of 1.5 μmol/kg. This dose of UCF 101 (ie, 1.5 μmol/kg) was used in all subsequent experiments. To further establish that inhibition of Omi/HtrA2 protease activity led to significant inhibition of apoptosis, the effect of UCF 101 on DNA ladder formation, a hallmark of apoptotic cell death, was determined. 30 minutes of ischemia followed by 3 hours of reperfusion resulted in significant DNA fragmentation, which was significantly reduced by treatment with UCF 101 before reperfusion[2].UCF 101 reduced the level of ERS and apoptosis in vivo. The ratio of p-GSK3β (Tyr216 to Ser9) increased in PD rats[3]. |
分子量 | 495.51 |
分子式 | C27H17N3O5S |
CAS No. | 313649-08-0 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 12.5 mg/mL (25.23 mM)
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
DMSO | 1 mM | 2.0181 mL | 10.0906 mL | 20.1812 mL | 50.4531 mL |
5 mM | 0.4036 mL | 2.0181 mL | 4.0362 mL | 10.0906 mL | |
10 mM | 0.2018 mL | 1.0091 mL | 2.0181 mL | 5.0453 mL | |
20 mM | 0.1009 mL | 0.5045 mL | 1.0091 mL | 2.5227 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
UCF 101 313649-08-0 Apoptosis Omi red Ucf-101 UCF-101 inhibit pro-apoptotic 543 HtrA2 MI/R Inhibitor cardioprotective His-Omi neuroprotective protease UCF101 Ucf101 fluorescence inhibitor