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Brassinolide

Brassinolide

产品编号 T3151   CAS 72962-43-7
别名: Brassin lactone, 24-Epibrassinolide, 芸苔素内酯

Brassinolide (Brassin lactone) 是一种植物生长调节剂,可调节植物细胞伸长。

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Brassinolide Chemical Structure
Brassinolide, CAS 72962-43-7
规格 价格/CNY 货期 数量
1 mg ¥ 185 现货
5 mg ¥ 413 现货
10 mg ¥ 663 现货
25 mg ¥ 1,070 现货
50 mg ¥ 1,490 现货
100 mg ¥ 2,230 现货
1 mL * 10 mM (in DMSO) ¥ 438 现货
其他形式的 Brassinolide:
千万补贴 助力科研
BCA蛋白浓度测定试剂盒限时半价
Venetoclax限时半价
产品目录号及名称: Brassinolide (T3151)
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纯度: 99.62%
纯度: 98%
纯度: 98%
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天然产物信息
生物活性
化学信息
存储 & 溶解度
TCMIP信息
参考文献
植物来源
结构类型
产品描述 Brassinolide (Brassin lactone) is a plant hormone isolated from rapeseed (Brassica napus) promoting stem elongation and cell division.
体外活性 Brassinolide is a plant sterol isolated from the pollen of rape (Brassica napus L.) initially. Brassinolide can induce a time and concentration-dependent cytotoxicity in PC-3 cells. The mode of cell death appears to be predominately apoptosis, as shown by flow-cytometric analysis, fluorescence and transmission electron microscopes. Caspase-3 activity is obviously increased after Brassinolide treatment. Western blot studies indicate that treatment with Brassinolide triggered a time-dependent decrease in the expression of anti-apoptotic protein Bcl-2, which suggests that Brassinolide can induce cytotoxicity in PC-3 cells by triggering apoptosis. Brassinolide might therefore be a promising candidate for the treatment of prostate cancer[1]. Brassinolide is a plant growth modulator, on multidrug resistance (MDR) of human T lymphoblastoid cell line CCRF-VCR 1000 which is obtained by progressively addition of vincristine (VCR) to sensitive CCRF-CEM cells, and to explore preliminarily the mechanism of reversing action. After treatment of Brassinolide under the concentration of 0.001-10 μg/mL, the resistance of CCRF-VCR is reversed partly with the reversing folds respectively as 4.4-11.6. The intracellular accumulation of rhodamine 123 is significantly reduced in the resistant cells. After treatment of Brassinolide, the accumulation increased, the level of fluorescent dye is situated between resistant cells and sensitive cells. No alteration of the catalytic activity of topoisomerase II is found among three groups. The level of protein expression of p53 in resistant cells is higher than that of sensitive cells. After Brassinolide treatment, the expression of p53 in CCRF-VCR cells restored to the level of sensitive cells. Brassinolide can effectively reverse the resistance of CCRF-VCR cells by inhibiting the effusion of drug transported by P-glucoprotein. To down regulate the abnormal expression of p53 maybe one of the mechanisms of reversing MDR for Brassinolide[2].
细胞实验 Brassinolide is dissolved in DMSO and stored, and then diluted with appropriate medium before use[2]. MTT method is used to detect the resistant factor of resistant cell line and the reversing fold after addition of Brassinolide. The intracellular accumulation of rhodamine 123, a fluorescent dye transported by P-glycoprotein is detected by flow cytometry, the catalytic activity of topoisomerase II is assessed by Sulliven method to find the effect of Brassinolide on resistance. The protein expression of p53 is measured using Western blotting in the sensitive cells and resistant cells to explore the effect of Brassinolide[2].
别名 Brassin lactone, 24-Epibrassinolide, 芸苔素内酯
分子量 480.68
分子式 C28H48O6
CAS No. 72962-43-7

存储

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

溶解度

DMSO: 60 mg/mL (124.82 mM)

溶液配制表

可选溶剂 浓度 体积 质量 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.0804 mL 10.4019 mL 20.8039 mL 52.0097 mL
5 mM 0.4161 mL 2.0804 mL 4.1608 mL 10.4019 mL
10 mM 0.208 mL 1.0402 mL 2.0804 mL 5.201 mL
20 mM 0.104 mL 0.5201 mL 1.0402 mL 2.6005 mL
50 mM 0.0416 mL 0.208 mL 0.4161 mL 1.0402 mL
100 mM 0.0208 mL 0.104 mL 0.208 mL 0.5201 mL
TCMIP相关数据
中药材来源及性味归经

中药材来源及性味归经

中药材名称 中药材拉丁名 归经
欧洲油菜 Brassica napus L. -

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TargetMol Library Books参考文献

1. Wu YD, et al. Brassinolide, a plant sterol from pollen of Brassica napus L., induces apoptosis in human prostate cancer PC-3 cells. Pharmazie. 2007 May;62(5):392-5. 2. Xian LJ, et al. Reversing effect of brassinolide on multidrug resistance of-CCRF-VCR12000 cells and a preliminary investigation on its mechanisms. Yao Xue Xue Bao. 2005 Feb;40(2):117-21.
Bergenin D-Pantothenic acid sodium Lenalidomide Sinomenine hydrochloride Sophocarpine monohydrate Xanthoangelol Fosbretabulin Disodium Ganoderenic acid D

相关化合物库

该产品包含在如下化合物库中:
烟草单体化合物库 Ro5类药天然产物库 人内源代谢化合物库 Plus 经典已知活性库 中药单体化合物库 植物来源化合物库 人代谢物化合物库 抗癌天然产物库 NO PAINS 化合物库 细胞凋亡化合物库

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请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。

母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。

体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。

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您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。

Keywords

Brassinolide 72962-43-7 Apoptosis Others Brassin lactone 24-Epibrassinolide Inhibitor inhibit 芸苔素内酯 inhibitor

 

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