Anti-NR4A1 Antibody (7O127) is a Rabbit antibody targeting NR4A1. Anti-NR4A1 Antibody (7O127) can be used in WB,ICC/IF,IHC.

IgG

Human,Mouse,Rat

1. Western blot analysis of NUR77 on rat brain cells lysates using anti-NUR77 antibody at 1/500 dilution.
2. ICC staining of NUR77 in Hela cells (green). 4% PFA fixed cells 20 minutes, washed with PBS. Cells were probed with the primary antibody (49510, 1/50) overnight at 4℃ washed with PBS. CoraLite488 Goat anti-Rabbit lgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).
3. ICC staining of NUR77 in 3T3-L1 cells (green). 4% PFA fixed cells 20 minutes, washed with PBS. Cells were probed with the primary antibody (49510, 1/50) overnight at 4℃ washed with PBS. CoraLite488 Goat anti-Rabbit lgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).
4. Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-NUR77 antibody (ET1703-97) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
5. Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-NUR77 antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
6. Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-NUR77 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
7. Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-NUR77 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
8. Immunohistochemical analysis of paraffin-embedded mouse ovarian tissue using anti-NUR77 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

WB: 1:500-1000; IHC: 1:50-200; ICC/IF: 1:50-200

Monoclonal

Unconjugated

Synthetic peptide within Human NUR77 aa 10-49 / 598

human