Powder: -20°C for 3 years | In solvent: -80°C for 1 year
RBC8是一种特异性 GTP 酶 RalA/RalB 抑制剂,通过抑制 Ral 与其效应物 RALBP1 的结合,对 GTP 酶 RhoA 和 Ras 没有抑制作用。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
1 mg | ¥ 367 | 现货 | ||
2 mg | ¥ 525 | 现货 | ||
5 mg | ¥ 943 | 现货 | ||
10 mg | ¥ 1,660 | 现货 | ||
25 mg | ¥ 2,790 | 现货 | ||
50 mg | ¥ 4,130 | 现货 | ||
100 mg | ¥ 5,880 | 现货 | ||
1 mL * 10 mM (in DMSO) | ¥ 996 | 现货 |
产品描述 | RBC8 is a specific GTPases RalA/RalB inhibitor by inhibiting the binding of Ral to its effector RALBP1, no inhibition on the GTPases RhoA and Ras. |
靶点活性 | Ral GTPase(H358 cell.):3.4 μM, GTPase(H2122 cell):3.5 μM |
体外活性 | 在负荷H358异种移植物的小鼠体内,RBC8(50 mg/kg,i.p.)可特异性抑制RalA和RalB,从而抑制肿瘤生长. |
体内活性 | 在活细胞中,RBC8k可诱导RalB–GDP中化学位移改变从而降低RalA的活化作用。在Ral依赖性H2122(IC50:3.5 μM)和H358(IC50:3.4 μM)中,RBC8引起anchorage-非依赖性生长抑制。 |
细胞实验 | Growth inhibition of human lung cancer cells by the compounds is measured under anchorage-independent conditions in soft agar. Cells are seeded into 6-well plates (coated with a base layer made of 2.0 ml of 1% low-melting-point agarose) at 15,000 cells per well in 3.0 mL of 0.4% low-melting-point agarose containing various concentration of drug. Two to four weeks (depending on the cell line) after incubation, the cells are stained with 1.0 mg ml?1 nitroblue tetrazolium, and colonies are counted under a microscope. The IC50 values are defined as the concentration of drug that resulted in a 50% reduction in colony number compared with the DMSO-treated control. (Only for Reference) |
分子量 | 424.45 |
分子式 | C25H20N4O3 |
CAS No. | 361185-42-4 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 42.4 mg/mL (100 mM)
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
DMSO | 1 mM | 2.356 mL | 11.78 mL | 23.5599 mL | 58.8998 mL |
5 mM | 0.4712 mL | 2.356 mL | 4.712 mL | 11.78 mL | |
10 mM | 0.2356 mL | 1.178 mL | 2.356 mL | 5.89 mL | |
20 mM | 0.1178 mL | 0.589 mL | 1.178 mL | 2.945 mL | |
50 mM | 0.0471 mL | 0.2356 mL | 0.4712 mL | 1.178 mL | |
100 mM | 0.0236 mL | 0.1178 mL | 0.2356 mL | 0.589 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
RBC8 361185-42-4 GPCR/G Protein MAPK GTPase Ras Inhibitor RBC-8 inhibit RBC 8 inhibitor