FGF-10 Protein, Human, Recombinant

产品编号 TMPY-01061

Fibroblast growth factor 10 (FGF10) is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. FGF10 exhibits mitogenic activity for keratinizing epidermal cells, but essentially no activity for fibroblasts, which is similar to the biological activity of FGF7. FGF10 plays an important role in the regulation of embryonic development, cell proliferation and cell differentiation. FGF10 is required for normal branching morphogenesis. It may play a role in wound healing. Defects in FGF10 are the cause of autosomal dominant aplasia of lacrimal and salivary glands (ALSG). ALSG has variable expressivity, and affected individuals may have aplasia or hypoplasia of the lacrimal, parotid, submandibular and sublingual glands and absence of the lacrimal puncta. The disorder is characterized by irritable eyes, recurrent eye infections, epiphora (constant tearing) and xerostomia (dryness of the mouth), which increases the risk of dental erosion, dental caries, periodontal disease and oral infections.

FGF-10 Protein, Human, Recombinant

产品编号 TMPY-01061

规格	价格	库存	数量
20 μg	¥ 1,650	现货
100 μg	¥ 5,410	现货

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常见问题解答

问蛋白能保证活性吗？

答我们有现货的产品，有活性数据的话会展示在官网生物活性那栏，没有数据的也不代表就一定没有活性，是我们还没有建立活性检测方法，没有进行测试，但是我们的蛋白都是优先按照有活性的方法去表达纯化的。

问重组蛋白产品是如何运输的？

答大部分重组蛋白产品为冻⼲粉，对温度不敏感，在室温下稳定，一般室温或蓝冰运输。对于液体类蛋白，一般采用⼲冰运输。如果您收到产品时发现冰盒已经融化，无需担心，蛋白可以正常使用，短时间内不会影响产品质量。如果特定蛋白在室温下不稳定，我们会提供相应的低温运输方式，以确保产品质量不受影响。

问重组蛋白有吗？

答您需要什么种属的蛋白呢，对表达系统和标签有要求吗，用于什么实验？

问如何将冻干的重组蛋白配置为储备液？

答在打开盖⼦之前，先使用 10000-12000 rpm 的转速进行离心，20-30 秒，将附着在管盖或管壁上的冻⼲粉收集到管底，以避免损失。大多数蛋白可通过加入无菌蒸馏水来重新溶解。如果需要除水以外的其他稀释剂，产品 COA 中会对其进行说明。一般储备液浓度建议不要低于 100 μg/mL，分装储存避免反复冻融。

问为什么某些重组蛋白溶液中还需要添加载体蛋白？

答载体蛋白通常被用于提高蛋白的稳定性，防止蛋白在冷冻或解冻过程中粘附在管壁上。塑料管对蛋白有一定的吸附能力，这可能导致蛋白和管壁之间难以分离，导致溶液中蛋白的实际浓度下降，从而影响其活性。为减少这种损失，在长期保存重组蛋白产品之前，建议添加常用的载体蛋白溶液，例如0.1% BSA（牛血清白蛋白）、5% HSA（人血清白蛋白）、10% FBS（胎牛血清）或 5% 海藻糖等。如果重组蛋白的浓度较低，强烈建议添加适量的载体蛋白。

产品信息

生物活性	1. Measured in a cell proliferation assay using BaF3 mouse pro-B cells transfected with human FGFR2b. The ED50 for this effect is typically 3-30 ng/mL. 2. Human lung cancer organoids were cultured with FGF2 (Cat#TMPY-00749), FGF4 (Cat#TMPY-05004), FGF7 (Cat#TMPY-00403), EGF (Cat#TMPY-03701), FGF10 (Cat#TMPY-01061), NOG (Cat#TMPY-02594), RSPO1 (Cat#TMPY-03626). 3. Human lung organoids were cultured with FGF2 (Cat#TMPY-00749), FGF4 (Cat#TMPY-05004), FGF7 (Cat#TMPY-00403), EGF (Cat#TMPY-03701), FGF10 (Cat#TMPY-01061), NOG (Cat#TMPY-02594), RSPO1 (Cat#TMPY-03626). 4. Human cholangiocarcinomas organoids were cultured with FGF2 (Cat#TMPY-00749), HGF (Cat#TMPY-02327), FGF7 (Cat#TMPY-00403), EGF (Cat#TMPY-03701), FGF10 (Cat#TMPY-01061), NOG (Cat#TMPY-02594), RSPO1 (Cat#TMPY-03626). 5. Human liver cancer organoids were cultured with FGF2 (Cat#TMPY-00749), HGF (Cat#TMPY-02327), FGF7 (Cat#TMPY-00403), EGF (Cat#TMPY-03701), FGF10 (Cat#TMPY-01061), TGFB1 (Cat#TMPY-02638), NOG (Cat#TMPY-02594), RSPO1 (Cat#TMPY-03626). 6. Human kidney cancer organoids were cultured with FGF2 (Cat#TMPY-00749), FGF7 (Cat#TMPY-00403), EGF (Cat#TMPY-03701), FGF10 (Cat#TMPY-01061), NOG (Cat#TMPY-02594), RSPO1 (Cat#TMPY-03626). 7. Human kidney organoids were cultured with FGF7 (Cat#TMPY-00403), EGF (Cat#TMPY-03701), FGF10 (Cat#TMPY-01061), NOG (Cat#TMPY-02594), RSPO1 (Cat#TMPY-03626), HGF (Cat#TMPY-02327), FGF4 (Cat#TMPY-05004). 8. Human gastric cancer organoids were cultured with EGF (Cat#TMPY-03701), FGF10 (Cat#TMPY-01061), NOG (Cat#TMPY-02594), RSPO1 (Cat#TMPY-03626). 9. Human stomach organoids organoids were cultured with EGF (Cat#TMPY-03701), FGF10 (Cat#TMPY-01061), NOG (Cat#TMPY-02594), RSPO1 (Cat#TMPY-03626). 10. Human small intestinal organoids were cultured with IL22 (Cat#TMPY-03490), FGF10 (Cat#TMPY-01061), EGF (Cat#TMPY-03701), NOG (Cat#TMPY-02594).
产品描述	Fibroblast growth factor 10 (FGF10) is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. FGF10 exhibits mitogenic activity for keratinizing epidermal cells, but essentially no activity for fibroblasts, which is similar to the biological activity of FGF7. FGF10 plays an important role in the regulation of embryonic development, cell proliferation and cell differentiation. FGF10 is required for normal branching morphogenesis. It may play a role in wound healing. Defects in FGF10 are the cause of autosomal dominant aplasia of lacrimal and salivary glands (ALSG). ALSG has variable expressivity, and affected individuals may have aplasia or hypoplasia of the lacrimal, parotid, submandibular and sublingual glands and absence of the lacrimal puncta. The disorder is characterized by irritable eyes, recurrent eye infections, epiphora (constant tearing) and xerostomia (dryness of the mouth), which increases the risk of dental erosion, dental caries, periodontal disease and oral infections.
种属	Human
表达系统	E. coli
标签	Tag Free
蛋白编号	O15520
别名	KGF2,fibroblast growth factor 10
蛋白构建	A DNA sequence encoding the mature form of human FGF10 (NP_004456.1) (Gln38-Ser208) was expressed. Predicted N terminal: Met
蛋白纯度	≥ 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.
分子量	19.46 kDa (predicted); 19.46 kDa (reducing conditions)
内毒素	< 5 EU/mg of the protein.
缓冲液	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
复溶方法	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
存储	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
运输方式	In general, Lyophilized powders are shipping with blue ice.
研究背景	Fibroblast growth factor 10 (FGF10) is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. FGF10 exhibits mitogenic activity for keratinizing epidermal cells, but essentially no activity for fibroblasts, which is similar to the biological activity of FGF7. FGF10 plays an important role in the regulation of embryonic development, cell proliferation and cell differentiation. FGF10 is required for normal branching morphogenesis. It may play a role in wound healing. Defects in FGF10 are the cause of autosomal dominant aplasia of lacrimal and salivary glands (ALSG). ALSG has variable expressivity, and affected individuals may have aplasia or hypoplasia of the lacrimal, parotid, submandibular and sublingual glands and absence of the lacrimal puncta. The disorder is characterized by irritable eyes, recurrent eye infections, epiphora (constant tearing) and xerostomia (dryness of the mouth), which increases the risk of dental erosion, dental caries, periodontal disease and oral infections.

FGF-10 Protein, Human, Recombinant

FGF-10 Protein, Human, Recombinant

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