Anti-APP Antibody (3L659)

Name: Anti-APP Antibody (3L659)
Brand: TargetMol
SKU: TMAH-00073
Rating: 4.5 (1 reviews)

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产品编号 TMAH-00073

别名 APP695, Amyloid Precursor Protein 695, Amyloid Precursor

Anti-APP Antibody (3L659) 是一种 Mouse 抗体，靶向 APP。Anti-APP Antibody (3L659) 可用于 ELISA, WB, IHC, IF。

Anti-APP Antibody (3L659)

还可以

产品编号 TMAH-00073 别名 APP695, Amyloid Precursor Protein 695, Amyloid Precursor

Anti-APP Antibody (3L659) 是一种 Mouse 抗体，靶向 APP。Anti-APP Antibody (3L659) 可用于 ELISA, WB, IHC, IF。

规格	价格	库存	数量
50 μg	¥ 1,300	5日内发货
100 μg	¥ 2,195	5日内发货

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产品描述	Anti-APP Antibody (3L659) is a Mouse antibody targeting APP. Anti-APP Antibody (3L659) can be used in ELISA, WB, IHC, IF.
别名	APP695, Amyloid Precursor Protein 695, Amyloid Precursor
Ig Type	IgG2b
克隆号	3L659
交叉反应	Human, Mouse
验证活性	1. Western blot -All lanes: Amyloid beta A4 antibody at 1µg/ml -Lane 1: Mouse heart tissue -Lane 2: Mouse kidney tissue -Lane 3: Mouse lung tissue -Secondary: Goat polyclonal to Mouse IgG at 1/15000 dilution -Predicted band size: 30, 35, 72, 80 kDa -Observed band size: 30, 35, 72 kDa 2. Immunohistochemistry of paraffin-embedded human brain tissue using TMAH-00073 at dilution of 1:200 3. Immunofluorescent analysis of Hela cells using TMAH-00073 at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) 4. Immunofluorescent analysis of HepG2 cells using TMAH-00073 at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
应用	ELISA WB IHC IF
抗体种类	Monoclonal
宿主来源	Mouse
亚细胞定位	Cell membrane; Single-pass type I membrane protein. Membrane; Single-pass type I membrane protein. Perikaryon. Cell projection, growth cone. Membrane, clathrin-coated pit. Early endosome. Cytoplasmic vesicle.; [C83]: Endoplasmic reticulum. Golgi apparatus. Early endosome.; [C99]: Early endosome.; [Soluble APP-beta]: Secreted.; [Amyloid-beta protein 42]: Cell surface.; [Gamma-secretase C-terminal fragment 59]: Nucleus. Cytoplasm.
构建方式	Hybridoma Monoclonal Antibody
纯化方式	Protein G purified
性状	Liquid
缓冲液	Preservative: 0.03% Proclin 300. Constituents: 50% Glycerol, 0.01M PBS, PH 7.4.
纯度	>95%
研究背景	Functions as a cell surface receptor and performs physiological functions on the surface of neurons relevant to neurite growth, neuronal adhesion and axonogenesis. Interaction between APP molecules on neighboring cells promotes synaptogenesis. Involved in cell mobility and transcription regulation through protein-protein interactions. Can promote transcription activation through binding to APBB1-KAT5 and inhibits Notch signaling through interaction with Numb. Couples to apoptosis-inducing pathways such as those mediated by G(O) and JIP. Inhibits G(o) alpha ATPase activity. Acts as a kinesin I membrane receptor, mediating the axonal transport of beta-secretase and presenilin 1. By acting as a kinesin I membrane receptor, plays a role in axonal anterograde transport of cargo towards synapes in axons. Involved in copper homeostasis/oxidative stress through copper ion reduction. In vitro, copper-metallated APP induces neuronal death directly or is potentiated through Cu(2+)-mediated low-density lipoprotein oxidation. Can regulate neurite outgrowth through binding to components of the extracellular matrix such as heparin and collagen I and IV. The splice isoforms that contain the BPTI domain possess protease inhibitor activity. Induces a AGER-dependent pathway that involves activation of p38 MAPK, resulting in internalization of amyloid-beta peptide and leading to mitochondrial dysfunction in cultured cortical neurons. Provides Cu(2+) ions for GPC1 which are required for release of nitric oxide (NO) and subsequent degradation of the heparan sulfate chains on GPC1. Amyloid-beta peptides are lipophilic metal chelators with metal-reducing activity. Bind transient metals such as copper, zinc and iron. In vitro, can reduce Cu(2+) and Fe(3+) to Cu(+) and Fe(2+), respectively. Amyloid-beta protein 42 is a more effective reductant than amyloid-beta protein 40. Amyloid-beta peptides bind to lipoproteins and apolipoproteins E and J in the CSF and to HDL particles in plasma, inhibiting metal-catalyzed oxidation of lipoproteins. APP42-beta may activate mononuclear phagocytes in the brain and elicit inflammatory responses. Promotes both tau aggregation and TPK II-mediated phosphorylation. Interaction with overexpressed HADH2 leads to oxidative stress and neurotoxicity. Also binds GPC1 in lipid rafts. Appicans elicit adhesion of neural cells to the extracellular matrix and may regulate neurite outgrowth in the brain. The gamma-CTF peptides as well as the caspase-cleaved peptides, including C31, are potent enhancers of neuronal apoptosis. N-APP binds TNFRSF21 triggering caspase activation and degeneration of both neuronal cell bodies (via caspase-3) and axons (via caspase-6).