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Anti-SEMA7A Polyclonal Antibody
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产品编号 TMAB-01676
Anti-SEMA7A Polyclonal Antibody 是一种 Rabbit 抗体，靶向 SEMA7A。Anti-SEMA7A Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,IF。
Anti-SEMA7A Polyclonal Antibody
Anti-SEMA7A Polyclonal Antibody
还可以产品编号 TMAB-01676
Anti-SEMA7A Polyclonal Antibody 是一种 Rabbit 抗体，靶向 SEMA7A。Anti-SEMA7A Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,IF。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,160 | 5日内发货 | |
| 100 μL | ¥ 1,960 | 5日内发货 | |
| 200 μL | ¥ 2,780 | 5日内发货 |
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产品介绍
生物活性
抗原信息
| 产品描述 | Anti-SEMA7A Polyclonal Antibody is a Rabbit antibody targeting SEMA7A. Anti-SEMA7A Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
| Ig Type | IgG |
| 交叉反应 | Human (predicted:Mouse,Rat,Dog,Cow) |
| 验证活性 | 1. Paraformaldehyde-fixed, paraffin embedded (human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (CD108) Polyclonal Antibody, Unconjugated (TMAB-01676) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 2. 25 μg total protein per Lane of various lysates probed with CD108 polyclonal antibody, unconjugated (TMAB-01676) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at RT for 60 min. |
| 应用 | |
| 推荐剂量 | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| 抗体种类 | Polyclonal |
| 宿主来源 | Rabbit |
| 亚细胞定位 | Cell membrane; Lipid-anchor, GPI-anchor; Extracellular side. Note=Detected in a punctate pattern on the cell membrane of basal and supra-basal skin keratinocytes. |
| 构建方式 | Hybridoma Polyclonal Antibody |
| 纯化方式 | Protein A purified |
| 性状 | Liquid |
| 缓冲液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究背景 | Semaphorin 7a is required for central and peripheral axon growth and is required for proper axon tract formation during embryonic development. Semaphorin 7a is also involved in modulating immune function. Semaphorin 7a expression is highest in the brain and increases in late embryonic and postnatal stages. The Semaphorins constitute a large family of secreted and membrane-tethered cell signaling molecules. They have functions in neural development, immunology, cardiac growth, vascular development, lung morphogenesis and axial bone patterning. Semaphorins are defined by the presence of a conserved Sema domain at the N-terminus. These proteins can be classified into eight classes depending on their structure and species origin. Classes 3 through 7 are found in vertebrates; class 3 are secreted, class 7 are GPI-anchored, and class 4 through 6 are transmembrane proteins. |
| 免疫原 | KLH conjugated synthetic peptide: human Sema7A |
| 抗原种属 | Human |
| 基因ID | |
| Uniprot ID | |
| 研究领域 | Neurogenesis,Axonal Guidance Proteins,Blood |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
计算器
比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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