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顶部产品介绍抗原信息化学信息储存和溶解信息计算器

Anti-NR0B2 Polyclonal Antibody

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货号 TMAB-01262

别名 Small heterodimer partner, SHP-1, Shp1, SHP, Orphan nuclear receptor SHP, Nuclear receptor subfamily 0, group B, member 2a, Nuclear receptor subfamily 0 group B member 2, Nr0b2a, NR0B2

Anti-NR0B2 Polyclonal Antibody 是一种 Rabbit 抗体，靶向 NR0B2。Anti-NR0B2 Polyclonal Antibody 可用于 IF,IHC-Fr,IHC-P,WB。

Anti-NR0B2 Polyclonal Antibody

Anti-NR0B2 Polyclonal Antibody

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货号 TMAB-01262 别名 Small heterodimer partner, SHP-1, Shp1, SHP, Orphan nuclear receptor SHP, Nuclear receptor subfamily 0, group B, member 2a, Nuclear receptor subfamily 0 group B member 2, Nr0b2a, NR0B2

Anti-NR0B2 Polyclonal Antibody 是一种 Rabbit 抗体，靶向 NR0B2。Anti-NR0B2 Polyclonal Antibody 可用于 IF,IHC-Fr,IHC-P,WB。

规格价格库存数量
50 μL
¥ 1,165
5日内发货
100 μL
¥ 1,975
5日内发货
200 μL
¥ 2,795
5日内发货
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产品介绍

生物活性
产品描述
Anti-NR0B2 Polyclonal Antibody is a Rabbit antibody targeting NR0B2. Anti-NR0B2 Polyclonal Antibody can be used in IF,IHC-Fr,IHC-P,WB.
别名Small heterodimer partner, SHP-1, Shp1, SHP, Orphan nuclear receptor SHP, Nuclear receptor subfamily 0, group B, member 2a, Nuclear receptor subfamily 0 group B member 2, Nr0b2a, NR0B2
Ig Type
IgG
反应种属
Mouse,Rat (predicted:Human,Dog,Cow,Horse,Rabbit)
验证活性
1. Tissue/cell: mouse spleen tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-NROB2 Polyclonal Antibody, Unconjugated (TMAB-01262) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
2. Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (NR0B2) Polyclonal Antibody, Unconjugated (TMAB-01262) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded (rat lung); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (NR0B2) Polyclonal Antibody, Unconjugated (TMAB-01262) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
4. Sample:
Lane 1: Rat Kidney tissue lysates
Lane 2: Rat Small intestine tissue lysates
Primary: Anti-NR0B2 (TMAB-01262) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 28 kDa
Observed band size: 28 kDa
verifiedActivityverifiedActivityverifiedActivityverifiedActivity
应用IFIHC-FrIHC-PWB
推荐剂量
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
抗体种类
Polyclonal
宿主来源Rabbit
亚细胞定位Nucleus. Cytoplasm. Note=Colocalizes with NEUROD1 in the nucleus.
组织特异性Liver. Low levels of expression were detected in heart and pancreas.
构建方式Polyclonal Antibody
纯化方式Protein A purified
性状Liquid
缓冲液0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
浓度1 mg/mL
研究背景SHP is an orphan nuclear receptor containing the dimerization and ligand-binding domains found in other nuclear receptors, but lacking the conserved DNA binding domain. SHP is specifically expressed in liver and other tissues, including fetal liver and adrenal gland, as well as adult spleen and small intestine. In addition, SHP is highy expressed in the murine macrophage cell line RAW 264.7 but suppressed by oxLDL and 13-HODE, which is a ligand for PPARg. SHP interacts with nuclear receptors, including thyroid receptor, retinoic acid receptors (RAR and RXR) and estrogen receptors (ERa and ERb). SHP functions as a negative regulator of these receptors by at least three mechanisms: inhibition of DNA binding via dimerization, direct antagonism of coactivator function through competition and possibly transrepression via recruitment of putative corepressors. In oxLDL-treated, resting macrophage cells, SHP acts as a transcription coactivator of NFkB, suggesting that SHP is a modulatory component in the regulation of the transcriptional activities of NFkB. Lastly, negative feedback regulation of a hepatic bile acid transporter, NTCP, is controlled by bile acid-activated FXR via induction of SHP to protect the hepatocyte from bile acid-mediated damage in cholestatic conditions.
抗原信息
免疫原
KLH conjugated synthetic peptide: human NR0B2
抗原种属
Human
基因名称
NR0B2
基因ID
8431
蛋白名称
Nuclear receptor subfamily 0 group B member 2
Uniprot ID
Q15466
研究领域
Retinoic & Retinoid,Lipid metabolism,Retinoic & Retinoid
功能
Acts as a negative regulator of receptor-dependent signaling pathways. Specifically inhibits transactivation of the nuclear receptor with whom it interacts.
化学信息
分子量Theoretical: 28 kDa.
储存&溶解度
储存方式Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
运输方式Shipping with blue ice.
存储store at low temperature | store at -20°C

计算器

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