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Anti-MCP-1/CCL2 Polyclonal Antibody

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Anti-MCP-1/CCL2 Polyclonal Antibody
产品编号 TMAB-01115

Anti-MCP-1/CCL2 Polyclonal Antibody 是一种 Rabbit 抗体,靶向 MCP-1/CCL2。Anti-MCP-1/CCL2 Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,IF。

Anti-MCP-1/CCL2 Polyclonal Antibody

Anti-MCP-1/CCL2 Polyclonal Antibody

Rating icon 还可以
Anti-MCP-1/CCL2 Polyclonal Antibody
产品编号 TMAB-01115

Anti-MCP-1/CCL2 Polyclonal Antibody 是一种 Rabbit 抗体,靶向 MCP-1/CCL2。Anti-MCP-1/CCL2 Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,IF。

规格价格库存数量
50 μL
¥ 1,175
5日内发货
100 μL
¥ 1,970
5日内发货
200 μL
¥ 2,790
5日内发货
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产品介绍

生物活性
产品描述
Anti-MCP-1/CCL2 Polyclonal Antibody is a Rabbit antibody targeting MCP-1/CCL2. Anti-MCP-1/CCL2 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Ig Type
IgG
交叉反应
Rat
验证活性
1. Tissue/cell: mouse kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-MCP-1 Polyclonal Antibody, Unconjugated (TMAB-01115) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
2. Paraformaldehyde-fixed, paraffin embedded (rat lung); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (MCP1) Polyclonal Antibody, Unconjugated (TMAB-01115) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
应用
推荐剂量
WB: 1:500-2000; IHC-P: 1:400-800; IHC-Fr: 1:400-800; IF: 1:100-500
抗体种类
Polyclonal
宿主来源Rabbit
亚细胞定位Secreted.
构建方式Hybridoma Polyclonal Antibody
纯化方式Protein A purified
性状Liquid
缓冲液0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
研究背景Chemotactic factor that attracts monocytes and basophils but not neutrophils or eosinophils. Augments monocyte anti-tumor activity. Has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis or atherosclerosis. May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis. Monomer or homodimer; in equilibrium. Binds to CCR2 and CCR4. Is tethered on endothelial cells by glycosaminoglycan (GAG) side chains of proteoglycans. Processing at the N-terminus can regulate receptor and targetcell selectivity. Deletion of the N-terminal residue converts it from an activator of basophil to an eosinophil chemoattractant. Genetic variations in CCL2 determine Mycobacterium tuberculosis susceptibility Belongs to the intercrine beta (chemokine CC) family. The MCP-1( the monocyte chemotactic protein1) has many pits that regulates the function toward sex to turn to record the factor, it can induce to have relation with inflammation with the immunity various genes turn to record.The immunity MCP-1 set turns the male signal to mainly locate the afterbirth syrup inside.
抗原信息
免疫原
KLH conjugated synthetic peptide: rat MCP-1
抗原种属
Rat
基因ID
Uniprot ID
研究领域
Response to hypoxia,Chemokines,Alpha Chemokines (CXC),Hypoxia,Heart disease
存储&运输
储存方式Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
运输方式Shipping with blue ice.

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