Anti-LMNB1 Polyclonal Antibody 2 is a Rabbit antibody targeting LMNB1. Anti-LMNB1 Polyclonal Antibody 2 can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF.

IgG

Rat,Mouse,Human

1. Sample:
Lane 1: Cerebrum (Mouse) Lysate at 40 μg
Lane 2: Pancreas (Mouse) Lysate at 40 μg
Lane 3: Testis (Mouse) Lysate at 40 μg
Lane 4: Stomach (Mouse) Lysate at 40 μg
Lane 5: Cerebrum (Rat) Lysate at 40 μg
Lane 7: Testis (Rat) Lysate at 40 μg
Lane 8: Stomach (Rat) Lysate at 40 μg
Lane 9: U937 (Human) Cell Lysate at 30 μg
Lane 10: Jurkat (Human) Cell Lysate at 30 μg
Lane 11: Molt-4 (Human) Cell Lysate at 30 μg
Lane 12: THP-1 (Human) Cell Lysate at 30 μg
Lane 13: Hela (Human) Cell Lysate at 30 μg
Primary:
Anti-Lamin B1 (TMAB-01078) at 1/1000 dilution
Anti-beta-Actin at 1/2000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 67-70 kDa
Observed band size: 65 kDa
2. Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Lamin B1) Polyclonal Antibody, Unconjugated (TMAB-01078) at 1:100 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Lamin B1) Polyclonal Antibody, Unconjugated (TMAB-01078) at 1:100 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded (human breast); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Lamin B1) Polyclonal Antibody, Unconjugated (TMAB-01078) at 1:100 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; ICC/IF: 1:50-200; IF: 1:100-500

Polyclonal

Recombinant Protein: human Lamin B1

Human

Lamins,Lamins,Lamin B1 - Nuclear,Lamins,Nuclear Envelope