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Anti-iNOS Polyclonal Antibody 2
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产品编号 TMAB-00971
Anti-iNOS Polyclonal Antibody 2 是一种 Rabbit 抗体，靶向 iNOS。Anti-iNOS Polyclonal Antibody 2 可用于 IHC-P,IHC-Fr,IF。
Anti-iNOS Polyclonal Antibody 2
Anti-iNOS Polyclonal Antibody 2
还可以产品编号 TMAB-00971
Anti-iNOS Polyclonal Antibody 2 是一种 Rabbit 抗体，靶向 iNOS。Anti-iNOS Polyclonal Antibody 2 可用于 IHC-P,IHC-Fr,IF。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,175 | 5日内发货 | |
| 100 μL | ¥ 1,965 | 5日内发货 | |
| 200 μL | ¥ 2,785 | 5日内发货 |
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产品介绍
生物活性
抗原信息
| 产品描述 | Anti-iNOS Polyclonal Antibody 2 is a Rabbit antibody targeting iNOS. Anti-iNOS Polyclonal Antibody 2 can be used in IHC-P,IHC-Fr,IF. |
| Ig Type | IgG |
| 交叉反应 | Rat (predicted:Mouse,Rabbit) |
| 验证活性 | Paraformaldehyde-fixed, paraffin embedded (Rat liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (iNOS) Polyclonal Antibody, Unconjugated (TMAB-00971) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining. |
| 应用 | |
| 推荐剂量 | IHC-P: 1:400-800; IHC-Fr: 1:400-800; IF: 1:100-500 |
| 抗体种类 | Polyclonal |
| 宿主来源 | Rabbit |
| 亚细胞定位 | Cytosol |
| 构建方式 | Hybridoma Polyclonal Antibody |
| 纯化方式 | Protein A purified |
| 性状 | Liquid |
| 缓冲液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究背景 | Nitric oxide (NO) is an inorganic, gaseous free radical that carries a variety of messages between cells. Vasorelaxation, neurotransmission and cytotoxicity can all be potentiated through cellular response to NO. NO production is mediated by members of the nitric oxide synthase (NOS) family. NOS catalyzes the oxidization of L-arginine to produce L-citrulline and NO. Two constitutive isoforms, brain or neuronal NOS (b or nNOS, type I) & endothelial cell NOS (eNOS, type III), and one inducible isoform (iNOS, type II), have been cloned. All NOS isoforms contain calmodulin, nicotinamide adenine dinucleotide phosphate (NADPH), flavin adenine dinucleotide (FAD), and flavin mononucleotide (FMN) binding domains. Nitric oxide synthase is expressed in liver, macrophages, hepatocytes, synoviocytes, stimulated glial cells and smooth muscle cells. Cytokines such as interferon-gamma (IFN), tumor necrosis factor (TNF), interleukin-1 and -2, and lipopolysaccarides (LPS) cause an increase in iNOS mRNA, protein, and activity levels. Protein kinase C-stimulating agents exhibit the same effect on iNOS activity. After cytokine induction, iNOS exhibits a delayed activity response which is then followed by a significant increase in NO production over a long period of time. Human iNOS is regulated by calcium/calmodulin (in contrast with mouse NOS2). |
| 免疫原 | KLH conjugated synthetic peptide: mouse NOS-2 |
| 抗原种属 | Mouse |
| 基因ID | |
| Uniprot ID | |
| 研究领域 | Cellular metabolic process,Response to hypoxia,Hypoxia,NOS |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
计算器
比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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