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Anti-BCL2 Polyclonal Antibody 3 是一种 Rabbit 抗体,靶向 BCL2。Anti-BCL2 Polyclonal Antibody 3 可用于 WB,IHC-P,IHC-Fr,ICC/IF,IF,ELISA。
Anti-BCL2 Polyclonal Antibody 3 是一种 Rabbit 抗体,靶向 BCL2。Anti-BCL2 Polyclonal Antibody 3 可用于 WB,IHC-P,IHC-Fr,ICC/IF,IF,ELISA。
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
50 μL | ¥ 1,160 | 5日内发货 | |
100 μL | ¥ 1,970 | 5日内发货 |
产品描述 | Anti-BCL2 Polyclonal Antibody 3 is a Rabbit antibody targeting BCL2. Anti-BCL2 Polyclonal Antibody 3 can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF,ELISA. |
Ig Type | IgG |
交叉反应 | Human,Mouse,Rat |
验证活性 | Sample: Lane 1: Spleen (Mouse) Lysate at 40 μg Lane 2: LympHnode (Mouse) Lysate at 40 μg Lane 3: LympHnode (Rat) Lysate at 40 μg Lane 4: MCF-7 (Human) Cell Lysate at 30 μg Lane 5: SiHa (Human) Cell Lysate at 30 μg Lane 6: Jurkat (Human) Cell Lysate at 30 μg Lane 7: A431 (Human) Cell Lysate at 30 μg Lane 8: Bone (Rat) Lysate at 40 μg Lane 9: Bone (Mouse) Lysate at 40 μg Lane 10: A549 (Human) Cell Lysate at 30 μg Lane 11: SH-SY5Y (Human) Cell Lysate at 30 μg Primary: Anti-Bcl-2 (TMAB-00198) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 26 kDa Observed band size: 26 kDa |
应用 | |
推荐剂量 | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-300; ICC/IF: 1:100-500; IF: 1:200-1000; ELISA: 1:10000 |
抗体种类 | Polyclonal |
宿主来源 | Rabbit |
亚细胞定位 | Mitochondrion outer membrane; Single-pass membrane protein. Nucleus membrane; Single-pass membrane protein. Endoplasmic reticulum membrane; Single-pass membrane protein. |
构建方式 | Hybridoma Polyclonal Antibody |
纯化方式 | Protein A purified |
性状 | Liquid |
缓冲液 | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Proclin300. |
研究背景 | The Bcl-2 gene was isolated at the chromosomal breakpoint of t(14;18)-bearing follicular B cell lymphomas(1,2).Bcl-2 blocks cell death following a variety of stimuli and confers a death-sparing effect to certain hematopoietic cell lines following growth factor withdrawal (3,5).Bcl-2 appears to function in several subcellular locations yet lacks any known motifs that would confer insight into its mechanism of action (6,7).A more recently identified protein,designated Bax p21(i.e., Bcl-associated X protein ),has extensive amino acid homology with Bcl-2 and both homodimerizes and forms heterodimers with Bcl-2(8). Overexpression of Bax accelerates apoptotic death induced by cytokine deprivation in an IL-3 dependent cell line and Bax also counters the death repressor activty of Bcl-2(8). |
免疫原 | KLH conjugated synthetic peptide: human Bcl-2 |
抗原种属 | Human |
基因ID | |
Uniprot ID | |
研究领域 | Cellular metabolic process,Response to hypoxia,Bcl 2 family,Metabolism,Bcl 2 family,Cell survival & death,Oncoproteins,Bcl2 Family,Apoptosis marker and proteins ELISA kits,Apoptosis,Hypoxia,Mitochondrial markers,Cancer,Mitochondrial |
储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
运输方式 | Shipping with blue ice. |
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