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Anti-ADGRE1 Polyclonal Antibody 2 是一种 Rabbit 抗体,靶向 ADGRE1。Anti-ADGRE1 Polyclonal Antibody 2 可用于 IHC-P,IHC-Fr,IF。
Anti-ADGRE1 Polyclonal Antibody 2 是一种 Rabbit 抗体,靶向 ADGRE1。Anti-ADGRE1 Polyclonal Antibody 2 可用于 IHC-P,IHC-Fr,IF。
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
50 μL | ¥ 1,170 | 5日内发货 | |
100 μL | ¥ 1,975 | 5日内发货 | |
200 μL | ¥ 2,780 | 5日内发货 |
产品描述 | Anti-ADGRE1 Polyclonal Antibody 2 is a Rabbit antibody targeting ADGRE1. Anti-ADGRE1 Polyclonal Antibody 2 can be used in IHC-P,IHC-Fr,IF. |
Ig Type | IgG |
交叉反应 | Mouse |
验证活性 | 1. Paraformaldehyde-fixed, paraffin embedded Mouse Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with ADGRE1 Polyclonal Antibody, Unconjugated (TMAB-00061) at 1:400 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. 2. Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with ADGRE1 Polyclonal Antibody, Unconjugated (TMAB-00061) at 1:400 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. 3. Paraformaldehyde-fixed, paraffin embedded Mouse Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with ADGRE1 Polyclonal Antibody, Unconjugated (TMAB-00061) at 1:400 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. 4. Paraformaldehyde-fixed, paraffin embedded Mouse Small Intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with ADGRE1 Polyclonal Antibody, Unconjugated (TMAB-00061) at 1:400 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. |
应用 | |
推荐剂量 | IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
抗体种类 | Polyclonal |
宿主来源 | Rabbit |
亚细胞定位 | Cell membrane. |
构建方式 | Hybridoma Polyclonal Antibody |
纯化方式 | Protein A purified |
性状 | Liquid |
缓冲液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
研究背景 | The epidermal growth factor (EGF)-TM7 family constitutes a group of class B G-protein coupled receptors, which includes CD97, EMR1 (EGF-like molecule containing mucin-like hormone receptor 1, designated F4/80 in mouse), EMR2, EMR3, FIRE, and ETL (1–3). These family members are characterized by an extended extracellular region with several N-terminal EGF domains, and are predominantly expressed on cells of the immune system (1–3). The EGF-TM7 protein family are encoded by a gene cluster on human chromosome 19p13 (1,3,4). The F4/80 molecule is solely expressed on the surface of macrophages and serves as a marker for mature macrophage tissues, including Kupffer cells in liver, splenic red pulp macrophages, brain microglia, gut lamina propria, and Langerhans cells in the skin (1). F4/80/EMR1 undergoes extensive N-linked glycosylation as well as some O-linked glycosylation (5,6). The function of F4/80/EMR1 is unclear, but it is speculated to be involved in macrophage adhesion events, cell migration, or as a G-protein coupled signaling component of macrophages. |
免疫原 | KLH conjugated synthetic peptide: human ADGRE1 |
抗原种属 | Human |
基因ID | |
Uniprot ID | |
研究领域 | Macrophage / Inflamm.,Other Cell Types |
储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
运输方式 | Shipping with blue ice. |
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