uPAR/PLAUR 重组蛋白, Human (His & Avi)

uPAR/PLAUR Protein, Human, Recombinant (His & Avi)

产品编号 TMPK-00226

The receptor (u-PAR) for urokinase plasminogen activator (u-PA) is a three-domain protein, GPI-anchored to the cell surface, which focuses the enzymatic activity of u-PA, and allows the cell surface activation of plasminogen.Regulation of the activity of u-PA is also mediated by u-PAR. uPAR/PLAUR Protein, Human, Recombinant (His & Avi) is expressed in HEK293 mammalian cells with C-His-Avi tag. The predicted molecular weight is 34.36 kDa and the accession number is Q03405-1.

规格	价格	库存
100 μg	¥ 1,320	5日内发货
500 μg	¥ 5,330	5日内发货
1 mg	¥ 8,900	5日内发货

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常见问题解答

问重组蛋白有吗？

答您需要什么种属的蛋白呢，对表达系统和标签有要求吗，用于什么实验？

问为什么重组蛋白的体积和质量与预期不符？

答蛋白冻干粉由蛋白原液冻干而来，冻干粉质量不等同于蛋白质量。由于冻⼲产品的体积大⼩受多种因素影响，包括冻⼲前缓冲液的组分、盐离⼦浓度以及产品本身的浓度等。冻⼲粉可能看上去很多，也可能肉眼不易观察到，这些都是正常现象，请您放心使用。在收到产品后，照常复溶、使用或分装储存即可。

问重组蛋白产品该如何保存？

答1) 存储条件:重组蛋白产品收到后，如不立即使用，应当保存在 -20℃ 至 -80℃ 的条件下。冻干粉可稳定保存 12 个月以上，液体类产品在 -80℃ 条件下可保存 6-12 个月。已复溶的重组蛋白样品需尽快用完，如果不能在一周内用完，需在 -20℃ 至 -80℃ 保存，可保存 3 个月以上\n2) 避免反复融冻:重组蛋白产品应注意避免反复冻融，建议将重组蛋白产品分装成多份。分装冻存时，建议选择含有一定浓度载体蛋白的溶液或培养基复溶产品。产品浓度不低于 100 ug/mL，且每管的体积不小于 20 uL。

问蛋白能保证活性吗？

答我们有现货的产品，有活性数据的话会展示在官网生物活性那栏，没有数据的也不代表就一定没有活性，是我们还没有建立活性检测方法，没有进行测试，但是我们的蛋白都是优先按照有活性的方法去表达纯化的。

问为什么某些重组蛋白溶液中还需要添加载体蛋白？

答载体蛋白通常被用于提高蛋白的稳定性，防止蛋白在冷冻或解冻过程中粘附在管壁上。塑料管对蛋白有一定的吸附能力，这可能导致蛋白和管壁之间难以分离，导致溶液中蛋白的实际浓度下降，从而影响其活性。为减少这种损失，在长期保存重组蛋白产品之前，建议添加常用的载体蛋白溶液，例如0.1% BSA（牛血清白蛋白）、5% HSA（人血清白蛋白）、10% FBS（胎牛血清）或 5% 海藻糖等。如果重组蛋白的浓度较低，强烈建议添加适量的载体蛋白。

产品信息

生物活性	Immobilized Human uPAR, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human Human PLAU, His Tag with the EC50 of 9.6ng/ml determined by ELISA.
产品描述	The receptor (u-PAR) for urokinase plasminogen activator (u-PA) is a three-domain protein, GPI-anchored to the cell surface, which focuses the enzymatic activity of u-PA, and allows the cell surface activation of plasminogen.Regulation of the activity of u-PA is also mediated by u-PAR. uPAR/PLAUR Protein, Human, Recombinant (His & Avi) is expressed in HEK293 mammalian cells with C-His-Avi tag. The predicted molecular weight is 34.36 kDa and the accession number is Q03405-1.
种属	Human
表达系统	HEK293 Cells
标签	C-His-Avi
蛋白编号	Q03405-1
别名	U-PAR,uPAR,PLAUR,MO3,CD87
蛋白构建	Leu23-Gly305
蛋白纯度	> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
分子量	34.36 kDa (predicted). Due to glycosylation, the protein migrates to 50-60 kDa based on Tris-Bis PAGE result.
内毒素	< 1 EU/μg by the LAL method.
缓冲液	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization.
复溶方法	Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
存储	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
运输方式	In general, Lyophilized powders are shipping with blue ice.
研究背景	The receptor (u-PAR) for urokinase plasminogen activator (u-PA) is a three-domain protein, GPI-anchored to the cell surface, which focuses the enzymatic activity of u-PA, and allows the cell surface activation of plasminogen.Regulation of the activity of u-PA is also mediated by u-PAR.

uPAR/PLAUR Protein, Human, Recombinant (His & Avi)

uPAR/PLAUR Protein, Human, Recombinant (His & Avi)

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