SCF Protein, Human, Recombinant (aa 1-189, His) is expressed in Baculovirus insect cells with His tag. The predicted molecular weight is 19.9 kDa and the accession number is P21583-1.
规格 | 价格/CNY | 货期 | 数量 | |
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100 μg | ¥ 2,930 | 现货 | ||
200 μg | ¥ 5,190 | 5日内发货 | ||
500 μg | ¥ 10,980 | 5日内发货 |
生物活性 |
Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is 1-5 ng/mL.
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产品描述 | SCF Protein, Human, Recombinant (aa 1-189, His) is expressed in Baculovirus insect cells with His tag. The predicted molecular weight is 19.9 kDa and the accession number is P21583-1. |
种属 | Human |
表达系统 | Baculovirus Insect Cells |
标签 | C-His |
蛋白编号 | P21583-1 |
别名 | DCUA, Kitl, FPHH, SCF, KL-1, SF, FPH2, MGF, SHEP7, C-kit ligand, KIT ligand |
蛋白构建 | The amino acid (Met 1-Ala 189) of Human SCF (P21583-1) extracellular domain was expressed, with a polyhistidine tag at the C-terminus. |
蛋白纯度 |
≥ 92 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC.
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分子量 | 19.9 kDa (predicted) |
内毒素 | < 1.0 EU/μg of the protein as determined by the LAL method. |
缓冲液 | Lyophilized from a solution filtered through a 0.22 μm filter, containing 20 mM Tris, 500 mM NaCl, pH 8.0.Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization. |
复溶方法 | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
存储 |
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted protein solutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
运输方式 |
In general, Lyophilized powders are shipping with blue ice. |
研究背景 | Similar to Kit ligand precursor (C-kit ligand), also known as Stem cell factor (SCF), Mast cell growth factor (MGF), or Hematopoietic growth factor KL. SCF/C-kit ligand is the ligand of the tyrosine-kinase receptor encoded by the KIT locus. This ligand is a pleiotropic factor that acts in utero in germ cell and neural cell development, and hematopoiesis, all believed to reflect a role in cell migration. In adults, it functions pleiotropically, while mostly noted for its continued requirement in hematopoiesis. SCF/C-kit ligand stimulates the proliferation of mast cells. This protein can augment the proliferation of both myeloid and lymphoid hematopoietic progenitors in bone marrow culture. It may act synergistically with other cytokines, probably interleukins SCF/C-kit ligand is the ligand for the tyrosine kinase receptor c-kit, which is expressed on both primitive and mature hematopoietic progenitor cells. In vitro, SCF/C-kit ligand synergizes with other growth factors, such as granulocyte colony-stimulating factor (G-CSF), granulocyte-macrophage colony-stimulating factor, and interleukin-3 to stimulate the proliferation and differentiation of cells of the lymphoid, myeloid, erythroid, and megakaryocytic lineages. In vivo, SCF/C-kit also synergizes with other growth factors and has been shown to enhance the mobilization of peripheral blood progenitor cells in combination with G-CSF. In phase I/II clinical studies administration of the combination of SCF and G-CSF resulted in a two- to threefold increase in cells that express the CD34 antigen compared with G-CSF alone. |
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SCF Protein, Human, Recombinant (aa 1-189, His) SHEP-7 DCUA Kitl FPH 2 FPHH SCF KL-1 SF FPH2 MGF SHEP7 FPH-2 KL 1 KL1 SHEP 7 C-kit ligand KIT ligand recombinant recombinant-proteins proteins protein