活性氧(ROS)探针

BODIPY 581 591 C11 是一种荧光放射探针，用于指示模型膜系统和活细胞中的脂质过氧化和抗氧化功效。

H2DCFDA (DCFH-DA) 属于绿色荧光染料，是一种检测细胞内活性氧 (ROS) 的探针 (Ex Em=488 525 nm)，具有细胞膜渗透性。

ADHP (10-Acetyl-3,7-dihydroxyphenoxazine) 是荧光过氧化物酶底物，其λex=530 nm，λem=590 nm。

1,3-Diphenylisobenzofuran (DPBF) 是一种荧光探针，对单线态氧(1O2) 的具有高特异性，可形成内过氧化物并分解成 1,2-dibenzoylbenzene，可用于活性氧生成的检测。

2',7'-Dichlorofluorescein diacetate (Diacetyldichlorofluorescein) 是一种可渗透细胞的荧光探针，可用于检测一氧化氮和活性氧以及确定总体氧化应激的程度。

Lucigenin (NSC-151912) 是化学发光探针，可用于检测内源性产生的超氧阴离子自由基在细胞的存在。

DHBS (DCHBS) 与 4-氨基安替比林和过氧化氢联合使用，能够用于偶联酶促反应中过氧化物酶的显色定量。它是 Trinder 试剂的组分，能够与过氧化物酶一起测量过氧化氢的产生。

Dihydrofluorescein diacetate (Diacetyldihydrofluorescein) 是一种荧光探针，用于测量无细胞系统和细胞模型中的氧化应激。

Hydroxyphenyl Fluorescein (HPF) 是一种用于检测高活性氧的细胞渗透性荧光染料，几乎没有内在荧光。Hydroxyphenyl Fluorescein 可应用于荧光酶标仪和流式细胞仪，可用于研究神经退行性变和衰老。其荧光波长：Ex Em=490 515 nm。

DAF-FM DA (Diaminofluorescein-FM diacetate) 是一种用于检测一氧化氮 (NO)和活性氮 （RNS） 水平的活性染料，可用于生物成像 (Ex Em=495 515 nm)。

HKPerox-1 is a yellow-emitting fluorescent probe with excellent selectivity and sensitivity toward H 2 O 2 . HKPerox-1 can be used for molecular imaging of endogenous H 2 O 2 in living cells.

DPPP is a probe that reacts stoichiometrically with hydroperoxides to yield the fluorescent molecule diphenyl-1-pyrenylphosphine oxide (DPPP-O). Plasma levels of lipid hydroperoxides of phosphatidylcholine, phosphatidylethanolamine, triglycerides, and cholesteryl esters have been measured by HPLC with a post column detection system using DPPP. DPPP has also been used as a fluorescent probe for the detection of low density lipoprotein and cellular oxidation. Fluorescence of DPPP-O can be monitor using excitation and emission wavelengths of 351 nm and 380 nm, respectively.

5(6)-Carboxy-2’,7’-dichlorofluorescein diacetate is an oxidant-sensitive and cell-permeable fluorescent probe.1Base hydrolysis of the ester bonds by intracellular esterases releases the acetate groups and the resulting compound, 5(6)-carboxy-2’,7’,-dichlorofluorescein, is oxidized by reactive oxygen species (ROS) and nitric oxide (NO) and displays excitation emission maxima of 504 and 530 nm, respectively, which can be quantified as a measure of oxidant production. 1.Hempel, S.L., Buettner, G.R., O’Malley, Y.Q., et al.Dihydrofluorescein diacetate is superior for detecting intracellular oxidants: Comparison with 2’,7’-dichlorodihydrofluorescein diacetate, 5(and 6)-carboxy-2’,7’-dichlorodihydrofluorescein diacetate, and dihydrorhodamine 123Free Radic. Biol. Med.27(1-2)146-159(1999)

FHZ is a fluorescent probe.

FBBBE is a derivatized fluorescein compound that is used to detect the production hydrogen peroxide (H2O2) by cells. Intracellular H2O2, but not extracellular H2O2, initiates the conversion of FBBBE to fluorescein, resulting in an increase in intracellular fluorescence (excitation emission maxima: 480 512 nm).

HKOH-1r is a highly sensitive and selective fluorescent probe which is used for detecting endogenous hydroxyl radicals in living cells.

IQ-R is a novel hypoxia-sensitive fluorescent probe that consists of an indolequinone unit coupled with a rhodol fluorophore.

HKOCl-3 is a fluorescent probe characterized by its high sensitivity and selectivity for hypochlorous acid detection, featuring an excitation wavelength (Ex) of 490 nm and an emission wavelength (Em) of 527 nm.

HKOH-1 is a highly sensitive and selective fluorescent probe for detecting endogenous hydroxyl radicals.