细胞活力检测

2-NBDG 是一种荧光指示剂 (λex=467 nm，λem=542 nm)，是一种荧光 D-葡萄糖类似物。2-NBDG 可用于监测活细胞和组织中的葡萄糖吸收，可以通过实时共聚焦、高分辨率或宽视场荧光显微镜和流式细胞术进行监测。

WST-8 是水溶性的四唑染料，与传统的基于 MTS 的测定法相比，基于 WST-8 的测定法灵敏度更高。

Thiazolyl Blue (MTT) 是一种比色剂，广泛用于测量细胞增殖，在活细胞中从黄色还原为紫色。

Evans blue (C.I. 23860) 是一种突触泡谷氨酸吸收的有效抑制剂，同时也是一种AMPA kainate 受体拮抗剂。

ATP-Red 1 是一种具有良好的膜通透性且多位点结合的可切换荧光探针，能够选择性地快速响应活细胞中ATP的细胞内浓度。

6-NBDG 是一种荧光葡萄糖类似物，可用作荧光探针。6-NBDG 可用于监测葡萄糖转运和摄取。6-NBDG 可用于检测富含巨噬细胞的动脉粥样硬化斑块。

Rose Bengal sodium 是一种合成的荧光素衍生物，主要成分是 4,5,6,7-tetrachloro-2,4,5,7-tetraiodo 荧光素，可以检测眼表干燥或受损的细胞，用作眼科诊断剂，具有抗病毒活性。它是一种 VGlut 和囊泡单胺转运蛋白抑制剂，VGlut 和 VMAT 的 Ki 分别为 19 和 64 nM。

Neutral Red 是含氮 pH 指示剂(pKi=6.8)，是类囊体内部酸化的指示剂。它能够将溶酶体染成红色。

Diphenyl Blue (Direct Blue 14) 是一种可用作偶氮染料组的参考染料。

Calcein Blue 是含有亚氨基二乙酸结构的香豆素衍生物，是不渗透膜的荧光染料，也是金属荧光变色指示剂。

CytoCalcein Violet 450 is a fluorogenic dye used to assess cell viability. It is a cell-permeable dye that, upon entering cells, is cleaved by intracellular esterases. CytoCalcein Violet 450 is retained by living cells and displays excitation emission maxima of 405 450 nm, respectively.

Calcein Orange Diacetate is a fluorogenic dye that is used to assess cell viability. It is hydrolyzed to the fluorescent probe Calcein Orange . Calcein Orange is retained by living cells and displays excitation emission maxima of 525 550 nm, respectively. Calcein Orange Diacetate is an orange-emitting variant of the green fluorophore Calcein AM , which has been used to evaluate cell viability and cytotoxicity.

XTT is a cell-impermeable, negatively charged tetrazolium dye that produces a water-soluble formazan when reduced at the cell surface by cellular-derived NADH and an electron mediator.1,2It is frequently used in colorimetric assays to measure cell proliferation, cytotoxicity, and apoptosis.3 1.Berridge, M.V., Tan, A.S., and Herst, P.M.Tetrazolium dyes as tools in cell biology: New insights into their cellular reductionBiotechnol. Ann. Rev.11127-152(2005) 2.Berridge, M.V., and Tan, A.S.Trans-plasma membrane electron transport: A cellular assay for NADH-and NADPH-oxidase based on extracellular, superoxide-mediated reduction of the sulfonated tetazolium salt WST-1Protoplasma20574-82(1998) 3.Sutherland, M.W., and Learmonth, B.A.The tetrazolium dyes MTS and XTT provide new quantitative assays for superoxide and superoxide dismutaseFree Radical Research27(3)283-289(1997)

Calcein Red AM is a fluorogenic dye that is used to assess cell viability. As the acetoxymethyl ester, Calcein Red AM is non-fluorescent but cell-permeant and is hydrolyzed to the fluorescent probe Calcein Red by intracellular esterases. Calcein Red is retained by living cells and displays excitation emission maxima of 560 574 nm, respectively. Calcein Red AM is a red-emitting variant of the green fluorophore calcein AM , which has been used to evaluate cell viability and cytotoxicity.

WST-3 is a sensitive tetrazolium reagent.

Ethidium monoazide bromide 是一种 DNA 嵌入荧光染料，可进入细胞膜受损的细菌。Ethidium monoazide bromide 可通过光活化与 DNA 共价连接。 Ethidium monoazide bromide 只染色死细胞。

Calcein tetraethyl ester, a fluorescent dye, is utilized in biological studies for its ability to penetrate cellular membranes into living cells. This property renders it valuable for assessing cell viability and short-term cell labeling.

CytoCalcein Violet 500 is a fluorogenic dye used to assess cell viability. It is a cell-permeable dye that, upon entering cells, is cleaved by intracellular esterases. CytoCalcein Violet 500 is retained by living cells and displays excitation emission maxima of 405 500 nm, respectively.