Powder: -20°C for 3 years | In solvent: -80°C for 1 year
TAME hydrochloride (Tos-Arg-OMe HCl) 是后期促进复合体(APC/C 或 APC)的抑制剂,可与 APC/C 结合并阻止其通过 Cdc20 和 Cdh1 活化,产生有丝分裂阻滞。它是一种 od 氨基酸衍生物。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
5 mg | ¥ 268 | 现货 | ||
10 mg | ¥ 393 | 现货 | ||
25 mg | ¥ 649 | 现货 | ||
50 mg | ¥ 813 | 现货 | ||
100 mg | ¥ 1,370 | 现货 | ||
500 mg | ¥ 3,430 | 现货 | ||
1 mL * 10 mM (in DMSO) | ¥ 297 | 现货 |
产品描述 | TAME hydrochloride (Tos-Arg-OMe HCl) is a kind od amino acid derivatives. |
激酶实验 | This clonal cell line, referred to as HEK 293 4.0-7 cells, are used in a high-throughput screening format to detect agonists and allosteric activators of the Ca2+?receptor. Changes in the concentration of cytoplasmic [Ca2+]i provide a quantitative and functional assessment of Ca2+ receptor activity in these cells and the results using this assay parallel those obtained using a homologous expression system of bovine parathyroid cells. On-line continuous measurements of fluorescence in fluo-3- or fura-2-loaded HEK 293 4.0-7 cells are obtained using a custom-built spectrofluorimeter or a fluorescence imaging plate reader instrument. NPS-2143 is incubated with cells for 1 minute before increasing the concentration of extracellular Ca2+?from 1.0 mM to 1.75 mM. NPS-2143 is tested individually at a concentration of 100 μg/mL (20 μM-80 μM) and those causing more than a 40% inhibition of the control response are considered to be biologically active. To determine the potencies (IC50) of NPS-2143 with biological activity, concentration-response curves are obtained and then, as an initial assessment of selectivity, the effects of NPS-2143 on [Ca2+]i evoked by other G protein-coupled receptors are examined at a concentration several times their IC50. Wild-type HEK 293 cells (and HEK 293 4.0-7 cells) express receptors for thrombin, bradykinin, and ATP, which couple to the mobilization of intracellular Ca2+. These responses can be studied to quickly assess any nonselective action of compounds on G protein-coupled receptors. Additional assays for selectivity include HEK 293 cells engineered to express receptors most homologous in sequence and topology to the Ca2+ receptor. These include native or chimeric receptors for various metabotropic glutamate and γ-aminobutyric acid type B receptors (GABABRs).Chimeric receptors are created using partial sequences of metabotropic glutamate receptors and Ca2+?receptors, engineered to couple to activation of phospholipase C and release of intracellular Ca2+?in HEK 293 cells. NPS-2143 lacking pan-activity are then subjected to structural modifications and their potencies and selectivities monitored using these HEK 293 4.0-7 cell assays in an iterative process. |
别名 | NA-P-甲苯磺酰-L-精氨酸甲酯盐酸盐, Tos-Arg-OMe HCl, TOS-ARG-OME hydrochloride |
分子量 | 378.87 |
分子式 | C14H23ClN4O4S |
CAS No. | 1784-03-8 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 37.9 mg/mL(100 mM)
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
DMSO | 1 mM | 2.6394 mL | 13.1971 mL | 26.3943 mL | 65.9857 mL |
5 mM | 0.5279 mL | 2.6394 mL | 5.2789 mL | 13.1971 mL | |
10 mM | 0.2639 mL | 1.3197 mL | 2.6394 mL | 6.5986 mL | |
20 mM | 0.132 mL | 0.6599 mL | 1.3197 mL | 3.2993 mL | |
50 mM | 0.0528 mL | 0.2639 mL | 0.5279 mL | 1.3197 mL | |
100 mM | 0.0264 mL | 0.132 mL | 0.2639 mL | 0.6599 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
TAME hydrochloride 1784-03-8 Cell Cycle/Checkpoint Metabolism Amino Acids and Derivatives APC TAME Hydrochloride NA-P-甲苯磺酰-L-精氨酸甲酯盐酸盐 Tos-Arg-OMe HCl inhibit Inhibitor TOS-ARG-OME Hydrochloride TAME TOS-ARG-OME hydrochloride Anaphase promoting complex inhibitor