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Didox

Didox

产品编号 T7525   CAS 69839-83-4
别名: 3,4-二羟基苯甲羟肟酸, NSC-324360

Didox (NSC-324360) 是一种合成的核糖核苷酸还原酶抑制剂,可降低患有痴呆症的 HIV 患者大脑中氧化损伤标志物的水平。

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Didox Chemical Structure
Didox, CAS 69839-83-4
规格 价格/CNY 货期 数量
1 mg ¥ 218 现货
5 mg ¥ 460 现货
10 mg ¥ 663 现货
25 mg ¥ 1,390 现货
50 mg ¥ 2,160 现货
100 mg ¥ 3,320 现货
500 mg ¥ 7,290 现货
1 mL * 10 mM (in DMSO) ¥ 492 现货
千万补贴 助力科研
BCA蛋白浓度测定试剂盒限时半价
Venetoclax限时半价
MG-132限时半价
产品目录号及名称: Didox (T7525)
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参考文献
产品描述 Didox (NSC-324360) is a synthetic ribonucleotide reductase (RR) inhibitor that has been found to reduce the levels of oxidative injury markers in the brains of HIV patients with dementia.
体外活性 Didox induced cell death and that this effect was suppressed by iron supplementation.?Cell treatments with didox caused changes of cellular iron content, TfR1 and ferritin levels comparable to those caused by the iron chelators, deferoxamine (DFO) and deferiprone (DFP).Didox is a bidentated iron chelator with two theoretical possible positions for the binding and among them that with the two hydroxyls of the catechol group acting as ligands is the more likely one. The iron chelating property of didox may contribute to its antitumor activity not only blocking the formation of the tyrosil radical on Tyr122 (such as HU) on RRM2 (essential for its activity) but also sequestering the iron needed by this enzyme and to the cell proliferation[1].
体内活性 Didox treatment of mouse bone marrow-derived mast cells (BMMC) reduced IgE-stimulated degranulation and cytokine production, including IL-6, IL-13, TNF and MIP-1a (CCL3)[2].
细胞实验 The cells were seeded in a 96-well plate (at a density of 2 × 10^3 cells for HA22T/VGH;?1.5 × 103 cells for HuH7) and exposed to various concentrations of didox and only HA22T/VGH also to hydroxyurea, DFO or DFP (0, 1, 10, 25, 50, 100, 200 and 500 μM) for 24, 48 and 72 h. In other experiments, HA22T/VGH were seeded in 96-well plates and treated with a single dose of didox, HU, DFO, DFP alone or in combination with increasing doses of FAC (25, 50, 100, 200 and 400 μM) for 48–72 h. In other type of treatment,?HA22T/VGH cells were or pre-treated for 16 h with a single dose of didox (200 μM) and then treated in combination with FAC (400–800 μM) or directly in combination didox-FAC for 48–72 h.Cell viability was evaluated with an MTT assay.?After the indicated time points and treatments, the supernatant was removed and 100 μL of the MTT solution (0.5 mg/mL) diluted in the cell medium was added to the wells.?After 3.5 h of incubation at 37 °C and 5% CO2, the MTT medium was removed and 75 μL of DMSO was added to each well.?Plates were shaken for 15 min at 37 °C until complete dissolution and absorbance was measured at 540 nm emission wavelengths.?Average percentage of cell viability at each concentration was calculated using Microsoft Excel 2016 software[1].
别名 3,4-二羟基苯甲羟肟酸, NSC-324360
分子量 169.13
分子式 C7H7NO4
CAS No. 69839-83-4

存储

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

溶解度

DMSO: 100 mg/mL (591.26 mM)

溶液配制表

可选溶剂 浓度 体积 质量 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 5.9126 mL 29.5631 mL 59.1261 mL 147.8153 mL
5 mM 1.1825 mL 5.9126 mL 11.8252 mL 29.5631 mL
10 mM 0.5913 mL 2.9563 mL 5.9126 mL 14.7815 mL
20 mM 0.2956 mL 1.4782 mL 2.9563 mL 7.3908 mL
50 mM 0.1183 mL 0.5913 mL 1.1825 mL 2.9563 mL
100 mM 0.0591 mL 0.2956 mL 0.5913 mL 1.4782 mL

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TargetMol Library Books参考文献

1. Asperti M, Cantamessa L, Ghidinelli S,et al.The Antitumor Didox Acts as an Iron Chelator in Hepatocellular Carcinoma Cells[1].Pharmaceuticals (Basel). 2019 Sep 2;12(3). 2. Mcleod J J A , Caslin H L , Spence A J , et al. Didox (3,4-dihydroxybenzohydroxamic acid) suppresses IgE-mediated mast cell activation through attenuation of NFκB and AP-1 transcription[J]. Cellular Immunology, 2017:S0008874917301442.
Docosanoic acid Norathyriol Bractoppin Filibuvir Deoxythymidine-5'-triphosphate trisodium Adenine SL327 RAD51 Inhibitor B02

相关化合物库

该产品包含在如下化合物库中:
抑制剂库 抗病毒库 抗癌化合物库 经典已知活性库 毒性化合物库 抗感染化合物库 已知活性化合物库 DNA 损伤和修复分子库 细胞周期化合物库 临床前化合物库

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Keywords

Didox 69839-83-4 Cell Cycle/Checkpoint DNA Damage/DNA Repair DNA/RNA Synthesis NSC 324360 Inhibitor 3,4-二羟基苯甲羟肟酸 inhibit NSC-324360 NSC324360 inhibitor

 

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