Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMPO (5,5-Dimethyl-1-pyrroline N-oxide) 是一种细胞渗透性亲水自旋诱捕剂,可用于超氧化物检测。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
10 mg | ¥ 213 | 现货 | ||
25 mg | ¥ 398 | 现货 | ||
50 mg | ¥ 659 | 现货 | ||
100 mg | ¥ 995 | 现货 | ||
200 mg | ¥ 1,490 | 现货 | ||
500 mg | ¥ 2,680 | 现货 |
产品描述 | DMPO (5,5-Dimethyl-1-pyrroline N-oxide) is a kind of water-soluble nitric oxide spin trap, which allows the measurement of oxygen-centered free radicals in biological systems at room temperature using electron spin resonance. It has a high reaction rate constant for superoxide and hydroxyl radicals and distinguishes simultaneously among a variety of important biologically generated free radicals. |
体外活性 | In vitro, It was demonstrated in red blood cells and in hamster V79 cells that the DMPO spin adducts of ·O2? and ·OH are metabolized very rapidly so that even if formed, they may not be detected in many experiments with cells [1]. |
体内活性 | When DMPO perfused the heart at 100 μm/L, it attenuated the development of reperfusion arrhythmias during the first 10 min of reperfusion and improved the functional recovery of the heart during reperfusion. Without treatment, 55% of hearts showed irreversible ventricular fibrillation, and this was completely prevented by DMPO, due to its function of traping·O2? and ·OH radicals [2]. |
别名 | 5,5-二甲基-1-吡咯啉-N-氧化物, 5,5-Dimethyl-1-pyrroline N-oxide |
分子量 | 113.16 |
分子式 | C6H11NO |
CAS No. | 3317-61-1 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 55 mg/mL (486.04 mM)
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
DMSO | 1 mM | 8.837 mL | 44.1852 mL | 88.3704 mL | 220.9261 mL |
5 mM | 1.7674 mL | 8.837 mL | 17.6741 mL | 44.1852 mL | |
10 mM | 0.8837 mL | 4.4185 mL | 8.837 mL | 22.0926 mL | |
20 mM | 0.4419 mL | 2.2093 mL | 4.4185 mL | 11.0463 mL | |
50 mM | 0.1767 mL | 0.8837 mL | 1.7674 mL | 4.4185 mL | |
100 mM | 0.0884 mL | 0.4419 mL | 0.8837 mL | 2.2093 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
DMPO 3317-61-1 Others inhibit Inhibitor 5,5-二甲基-1-吡咯啉-N-氧化物 5,5-Dimethyl-1-pyrroline N-oxide inhibitor