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TIM-3/KIM-3/HAVCR2 Protein, Human, Recombinant (hFc)

产品编号 TMPS-00135

TIM-3/KIM-3/HAVCR2 Protein, Human, Recombinant (hFc) is expressed in HEK293 Cells. The accession number is Q8TDQ0.

TIM-3/KIM-3/HAVCR2 Protein, Human, Recombinant (hFc)

产品编号 TMPS-00135

TIM-3/KIM-3/HAVCR2 Protein, Human, Recombinant (hFc) is expressed in HEK293 Cells. The accession number is Q8TDQ0.

规格	价格	库存	数量
50 μg	¥ 3,960	5日内发货
1 mg	¥ 21,600	5日内发货

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产品信息

生物活性	Assay #1: Immobilized TIM-3, hFc, Human at 0.5 μg/ml, the concentration of Anti-TIM3 mouse antibody (Genscript) that produces 50% optimal binding response is found to be approximately 5.0 ng/ml. Assay #2: Immobilized Galectin-9, His, Human at 0.5 μg/ml (100 µl/well) can bind TIM-3, hFc, Human with a linear range of 0.78-6.25 μg/ml.
产品描述	TIM-3/KIM-3/HAVCR2 Protein, Human, Recombinant (hFc) is expressed in HEK293 Cells. The accession number is Q8TDQ0.
种属	Human
表达系统	HEK293 Cells
标签	hFc
蛋白编号	Q8TDQ0
别名	TIMD-3,TIM-3,T-cell membrane protein 3,T-cell immunoglobulin mucin receptor 3,T-cell immunoglobulin and mucin domain-containing protein 3,Hepatitis A virus cellular receptor 2,HAVcr-2
蛋白构建	Ser22-Arg200
蛋白纯度	> 95% as determined by SDS-PAGE
分子量	60~65 kDa (Reducing conditions)
内毒素	< 0.2 EU/μg of protein as determined by the LAL method.
缓冲液	Lyophilized from a 0.2 μm filtered solution in PBS.
复溶方法	Reconstitute the lyophilized protein in sterile deionized water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
存储	Upon receiving, this product remains stable for up to 6 months at lower than -70°C. Upon reconstitution, the product should be stable for up to 1 week at 4°C or up to 3 months at -20°C. For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles.
运输方式	In general, Lyophilized powders are shipping with blue ice. Solutions are shipping with dry ice.
研究背景	T cell Ig- and mucin-domain-containing molecules (TIMs) are a family of transmembrane proteins expressed by various immune cells. TIM-3 is an inhibitory molecule that is induced following T cell activation. TIM-3 is expressed by exhausted T cells in the settings of chronic infection and cancer, and tumor-infiltrating T cells that co-express PD-1 and TIM-3 exhibit the most severe exhausted phenotype. Tumor-infiltrating dendritic cells also express TIM-3. TIM-3 expression on DCs was found to suppress innate immunity by reducing the immunogenicity of nucleic acids released by dying tumor cells. Research studies show that heterodimerization of TIM-3 with CEACAM-1 is critical for the inhibitory function of TIM-3, and co-blockade of TIM-3 and CEACAM-1 enhanced antitumor responses in a mouse model of colorectal cancer. Its binding to Galectin-9 induces a range of immunosuppressive functions which enhance immune tolerance and inhibit anti-tumor immunity. TIM-3 ligation attenuates CD8 + and Th1 cell responses and promotes the activity of Treg and myeloid derived suppressor cells. In addition, dendritic cell-expressed TIM-3 dampens inflammation by enabling the phagocytosis of apoptotic cells and the cross-presentation of apoptotic cell antigens.