Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Sulfacetamide sodium monohydrate 是一种磺胺类药物,用于治疗酒渣鼻和花斑糠疹。
产品描述 | Sulfacetamide sodium monohydrate is a sulfonamide antibiotic. It has been investigated for the treatment of rosacea and pityriasis versicolor. |
靶点活性 | DHPS:9.5 μM |
体外活性 | Sulfacetamide sodium monohydrate 抑制拟南芥DHPS,IC50为9.5 μM,pKa=5.4[1]。Sulfacetamide sodium monohydrate 对T-47D细胞具有抗增殖效应,这一效应独立于凋亡和细胞周期阻滞。Sulfacetamide sodium monohydrate 降低了p53/DRAM通路的表达,促进 Akt/mTOR 通路的上调,促进细胞保护性自噬。在48小时后,Sulfacetamide sodium monohydrate 对 T-47D 细胞的LD50为41 mM。Sulfacetamide处理的细胞使ATG5表达水平上升,增加在自噬通路中自噬体形成。在sulfathiazole 和 Sulfacetamide sodium monohydrate 诱导细胞自噬单不伴随细胞凋亡,且未在细胞周期的任何明确阶段发生阻滞作用。Sulfacetamide sodium monohydrate 通过DAPK独立通路触发T-47D细胞的自噬[2]。Sulfacetamide sodium monohydrate 是一种抗菌静止剂,对多种对磺胺药敏感的革兰氏阴性菌和革兰氏阳性菌活跃,包括链球菌、葡萄球菌、大肠杆菌、肺炎克雷伯菌、绿脓假单胞菌、沙门菌属、粪肠杆菌和诺卡菌属,这些细菌通常在皮肤的继发感染中被分离出来。Sulfacetamide还抑制了磷酸甘露糖异构酶(也称为磷酸甘露糖异构酶(PMI)),这被认为是鞭毛虫能量代谢的关键酶[3]。 |
体内活性 | 小鼠口服 Sulfacetamide sodium monohydrate 的LD50为16,500 mg/kg。其副作用包括红斑、中度肿胀、恶心、呕吐和头痛。接受磺胺滴眼液治疗眼部感染的艾滋病毒阳性患者会出现史蒂文斯-约翰逊综合征。然而,所有这些副作用都与口服或通过皮肤、粘膜和结膜吸收大量药物有关,而局部使用则不会产生强烈的副作用。 |
细胞实验 | Cells are cultured in RPMI medium 1640, supplemented with 10% FBS and 1% penicillin/streptomycin, in a humidified atmosphere of 5% carbon dioxide in air at 37°C. According to MTT assay, the LC50 of sulfathiazole and sulfacetamide after 48 h is determined as 6.5 mM and 41 mM, respectively. Doxorubicin and sodium salt of sulfadrugs are dissolved in culture medium to the final desired concentration based on the determined LC50 and filtered. Cells (at 80% confluency) are incubated with freshly prepared drugs for 48h in a humidified incubator before being trypsinized and washed with phosphate-buffer saline 3 times and stored at ?70°C. For cell viability assay, cells are seeded in at least triplicate wells for each concentration of drug per time at 1 × 104 cells/well in a 96-well plate. After 24h of seeding, the cells has grown to ~80% confluency. The medium is changed to that containing drugs at concentrations ranging from 0.0-50 mM. The concentration range for doxorubicin is 0-6 μM. After 24, 48 and 72 h, each well is filled with 25 μl MTT stock solution (4 mg/ml or 100 μg/well) and incubated for 3 h at 37°C. Formazan crystals are dissolved in 100 μl of dimethyl sulfoxide (DMSO) and quantified using a microplate reader at 570 nm. The MTT assays are performed at least 3 times for each drug and the percentage of surviving cells relative to control (untreated sample) is calculated.(Only for Reference) |
别名 | 磺胺乙酰钠水合物, Sulfacetamide sodium salt hydrate, Sulfacetamide sodium |
分子量 | 254.2 |
分子式 | C8H9N2NaO3S·H2O |
CAS No. | 6209-17-2 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 196.7 mM
H2O: 196.7 mM
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
DMSO / H2O | 1 mM | 3.9339 mL | 19.6696 mL | 39.3391 mL | 98.3478 mL |
5 mM | 0.7868 mL | 3.9339 mL | 7.8678 mL | 19.6696 mL | |
10 mM | 0.3934 mL | 1.967 mL | 3.9339 mL | 9.8348 mL | |
20 mM | 0.1967 mL | 0.9835 mL | 1.967 mL | 4.9174 mL | |
50 mM | 0.0787 mL | 0.3934 mL | 0.7868 mL | 1.967 mL | |
100 mM | 0.0393 mL | 0.1967 mL | 0.3934 mL | 0.9835 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
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