Powder: -20°C for 3 years | In solvent: -80°C for 1 year
SCH28080 是一种可逆且具有 K+ 竞争性的胃 H+/K+-ATP 酶 (H+/K+-ATPase) 抑制剂,IC50 值为 20 nM (家兔微粒体膜)。SCH28080 在体内是有效的酸分泌抑制剂,具有抗溃疡活性、抗分泌和细胞保护活性。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
1 mg | ¥ 328 | 现货 | ||
5 mg | ¥ 913 | 现货 | ||
10 mg | ¥ 1,490 | 现货 | ||
25 mg | ¥ 2,930 | 现货 | ||
50 mg | ¥ 4,390 | 现货 | ||
100 mg | ¥ 6,180 | 现货 | ||
1 mL * 10 mM (in DMSO) | ¥ 818 | 现货 |
产品描述 | SCH28080 is a reversible and K+-competitive inhibitor of gastric H+/K+-ATPase with an IC50 value of 20 nM (rabbit microsomal membrane).SCH28080 is a potent inhibitor of acid secretion in vivo with anti-ulcer activity, anti-secretory and cytoprotective activity. |
靶点活性 | H+, K+-ATPase, rabbit microsomal membranes:20 nM, H+, K+-ATPase:0.12 μM(Ki), Aminopyrine:0.029 μM, INS-1E cells:22.9 µM, INS-1E cells:15.3 µM |
体外活性 |
SCH28080 competitively hinders ATP hydrolysis stimulated by K+, with a Ki value of 0.12 μM.[1] SCH28080 effectively suppresses histamine-induced uptake of [14C]aminopyrine in isolated rabbit parietal cells, demonstrating an IC50 of 0.029 μM.[1] SCH28080 elicits a dose-dependent decrease in cell viability, with IC50 values of 22.9 µM and 15.3 µM following 2-hour and 24-hour treatments, respectively. And at concentration of 100 µM, cell viability drops below 10% as early as 2 hours.[2] SCH28080 induces apoptosis and exhibits cytotoxicity at higher doses.[2]. SCH28080 also impedes insulin secretion by activating IK ATP and inhibiting L-type voltage-gated Ca2+ channels, ultimately reducing cell viability and induces apoptosis/necrosis in a dose-dependent manner.[2] |
体内活性 | SCH28080 (20 mg/kg; i.p.) effectively attenuates gastric ulcers induced by pylorus ligation in rats.[3] |
分子量 | 277.32 |
分子式 | C17H15N3O |
CAS No. | 76081-98-6 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 90.0 mg/mL (324.5 mM), Sonication is recommended.
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
DMSO | 1 mM | 3.6059 mL | 18.0297 mL | 36.0594 mL | 90.1486 mL |
5 mM | 0.7212 mL | 3.6059 mL | 7.2119 mL | 18.0297 mL | |
10 mM | 0.3606 mL | 1.803 mL | 3.6059 mL | 9.0149 mL | |
20 mM | 0.1803 mL | 0.9015 mL | 1.803 mL | 4.5074 mL | |
50 mM | 0.0721 mL | 0.3606 mL | 0.7212 mL | 1.803 mL | |
100 mM | 0.0361 mL | 0.1803 mL | 0.3606 mL | 0.9015 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
SCH28080 76081-98-6 Membrane transporter/Ion channel Proton pump ATPase SCH-28080 SCH 28080 Inhibitor inhibitor inhibit